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Gene Expression Data
Assay Details
Assay
Reference: J:135698 Burlison JS, et al., Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol. 2008 Apr 1;316(1):74-86
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6695726
Gene symbol: Ptf1a
Gene name: pancreas specific transcription factor, 1a
Antibody: anti-Ptf1a
Results
Specimen 5B Ptf1a: embryonic day 10.5 (more )
Note: Triple labeled: green - Ptf1a; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Scattered 5B Ptf1a , 5B Merge Expression in pancreatic progenitor cells of dorsal pancreatic bud at E10.5 coinciding with Pdx1 and Ngn3 expression in scattered cells throughout pancreatic epithelium.
Specimen E11.5 +/+ not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: pancreas primordium Present Expression still coincides with that of Pdx1 in almost all pancreatic epithelial cells.
Specimen S1 Ptf1a E9.5: embryonic day 9.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: red - Ptf1a protein; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present Widespread S1_E9.5 Merge , S1_E9.5 Ptf1a Expression throughout dorsal and ventral pancreatic buds.
TS15: pancreas primordium ventral bud Present Widespread S1_E9.5 Merge , S1_E9.5 Ptf1a Expression throughout dorsal and ventral pancreatic buds.
Specimen S1 Ptf1a E10.5: embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: red - Ptf1a protein; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1_E10.5 Ptf1a , S1_E10.5 Merge
Specimen S1 Ptf1a E11.5: embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: red - Ptf1a protein; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS19: pancreas primordium Present S1_E11.5 Merge , S1_E11.5 Ptf1a
Specimen S1 Ptf1a E12.5: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: red - Ptf1a protein; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present Regionally restricted S1_E12.5 Merge , S1_E12.5 Ptf1a Expression in multipotent progenitor cells (MPCs) residing in distal tipe of branching epithelium at periphery of pancreas.
Specimen S1 Ptf1a E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: red - Ptf1a protein; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS21: pancreatic acinus Present S1_E13.5 Merge , S1_E13.5 Ptf1a From E13.5 on, expression coincident with Ptf1a in all acinar cells.
Specimen S1 Ptf1a E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: red - Ptf1a protein; green - Ptf1a reporter (assay MGI:6695715).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present S1_E15.5 Merge , S1_E15.5 Ptf1a From E13.5 on, expression coincident with Ptf1a in all acinar cells.
Specimen S2A Ptf1a: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: red - Ptf1a protein; blue - Pdx1 (assay MGI:6695727); green - Ptf1a reporter (assay MGI:6695715)
Structure Level Pattern Image Note
TS20: pancreas epithelium Present Regionally restricted S2A Ptf1a , S2A Merge Expression has begun to compartmentalize to distal tips of pancreatic epithelium.
Specimen S2B Ptf1a: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: red - Ptf1a protein; blue - Pdx1 (assay MGI:6695727); green - Ptf1a reporter (assay MGI:6695715)
Structure Level Pattern Image Note
TS20: pancreas Absent S2B Ptf1a , S2B Merge
Specimen S2C Ptf1a: embryonic day 10.5 (more )
Note: Triple labeled: green - Ptf1a; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted S2C Merge , S2C Pff1a
Specimen S3B/D: embryonic day 9.5 (more )
Note: Triple labeled: green - Ptf1a; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS15: pancreas primordium Present Regionally restricted S3B , S3D No expression in pre-pancreatic endoderm.
Specimen S4 Ptf1a: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS15: foregut epithelium Present S4_Ptf1a , S4_Merge Expression in foregut endoderm as early as E9.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory