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Gene Expression Data
Assay Details
Assay
Reference: J:206541 Engelhard C, et al., MEGF8 is a modifier of BMP signaling in trigeminal sensory neurons. Elife. 2013;2:e01160
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6451144
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament clone 2H3
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 1A control: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 1A Control
Specimen 1A mutant: embryonic day 11.5; Megf8m687Ddg/Megf8m687Ddg (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 1A Mutant In the mutant the ophthalmic projection of the nerve exhibits dramatic defasciculation and undergrowth; the maxillary and mandibular branches are largely unaffected.
Specimen 1 S1B L1775P/+: embryonic day 11.5; Megf8m687Ddg/Megf8+ (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 1 S1B left
Specimen 1 S1B L1775P/Trap: embryonic day 11.5; Megf8Gt(G037A09)Wrst/Megf8m687Ddg (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 1 S1B right In the mutant the ophthalmic projection of the nerve exhibits dramatic defasciculation and undergrowth; the maxillary and mandibular branches are largely unaffected.
Specimen 3A +/+: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 3A left
Specimen 3A -/-: embryonic day 11.5; Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 3A right In the mutant the ophthalmic projection of the nerve exhibits dramatic defasciculation and undergrowth; the maxillary and mandibular branches are largely unaffected.
Specimen 4A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: spinal nerve Present 4A
Specimen 4D: embryonic day 11.5; Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Structure Level Pattern Image Note
TS19: spinal nerve Present 4D In the mutant the spinal nerves are shorter.
Specimen 4C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: glossopharyngeal nerve Present 4C
TS19: vagus nerve Present 4C
Specimen 4F: embryonic day 11.5; Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Structure Level Pattern Image Note
TS19: glossopharyngeal nerve Present 4F In the mutant the nerve is slightly defasciculated and several wayward axons branch away from the main fiber bundle.
TS19: vagus nerve Present 4F In the mutant the nerve is slightly defasciculated and several wayward axons branch away from the main fiber bundle.
Specimen 4G flox/flox: embryonic day 11.5; Megf8tm1.2Ddg/Megf8tm1.2Ddg (more )
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 4G left
Specimen 4G flox/flox; cre: embryonic day 11.5; Megf8tm1.2Ddg/Megf8tm1.2Ddg, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 4G right In the mutant the ophthalmic projection of the nerve exhibits defasciculation, the severity of which varies, and undergrowth.
Specimen 5A left: embryonic day 10.5; Bmp4tm2Blh/Bmp4+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS17: cranial nerve Present 5A E10.5 Expression is seen in the developing ophthalmic projection of the trigeminal nerve.
Specimen 5A right: embryonic day 11.5; Bmp4tm2Blh/Bmp4+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5A E11.5 Expression is seen in the trigeminal nerve, including the frontal and nasociliary nerves and ophthalmic branch.
Specimen 5B +/+: embryonic day 11.5; Bmp4tm2Blh/Bmp4+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5B bottom row - left , 5B top row - left
Specimen 5B -/-: embryonic day 11.5; Bmp4tm2Blh/Bmp4+, Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5B bottom row - center , 5B top row - center In the mutant the ophthalmic projection of the nerve exhibits dramatic defasciculation and undergrowth.
Specimen 5B flox/flox; cre: embryonic day 11.5; Bmp4tm2Blh/Bmp4+, Megf8tm1.2Ddg/Megf8tm1.2Ddg, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5B top row - right , 5B bottom row - right In the mutant the ophthalmic projection of the nerve exhibits defasciculation, the severity of which varies, and undergrowth.
Specimen 5C left: embryonic day 11.5; Bmpr2tm1.1Enl/Bmpr2+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5C left
Specimen 5C right: embryonic day 11.5; Bmpr2tm1.1Enl/Bmpr2tm1.2Enl, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5C right In the mutant the ophthalmic branch of the nerve is stunted and branches prematurely; the maxillary and mandibular branches of the nerve are unaffected.
Specimen 5 S1A left: embryonic day 11.5; Bmp4tm2Blh/Bmp4+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5 S1A left
Specimen 5 S1A right: embryonic day 11.5; Bmp4tm2Blh/Bmp4+, Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: trigeminal nerve Present 5 S1A right In the mutant the ophthalmic projection of the nerve exhibits dramatic defasciculation and undergrowth.
Specimen 5 S1B left: embryonic day 11.5; Bmp4tm2Blh/Bmp4+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: spinal nerve Present 5 S1B left
TS19: dorsal root ganglion Present 5 S1B left
Specimen 5 S1B right: embryonic day 11.5; Bmp4tm2Blh/Bmp4+, Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: spinal nerve Present 5 S1B right In the mutant the spinal nerves are shorter.
TS19: dorsal root ganglion Present 5 S1B right
Specimen 5 S1C left: embryonic day 11.5; Bmp4tm2Blh/Bmp4+ (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: limb nerve Present 5 S1C left
Specimen 5 S1C right: embryonic day 11.5; Bmp4tm2Blh/Bmp4+, Megf8tm1.1Ddg/Megf8tm1.1Ddg (more )
Note: Double labeled: brown - Nefm; blue - Bmp4 (assay MGI:6451176)
Structure Level Pattern Image Note
TS19: limb nerve Present 5 S1C right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory