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Gene Expression Data
Assay Details
Assay
Reference: J:290315 Cheong A, et al., Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development. 2020 May 26;147(10):dev188714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6445617
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved caspase-3 (Asp175) (5A1E; #9664)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Assay notes: The antibody used in this assay recognizes the active (cleaved) form of the protein.
Results
Specimen 5H: embryonic day 7.5 (more )
Note: Littermate control; embryo is either +/+ or +/-. Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS11: conceptus Absent 5H'' , 5H This protein is a marker of apoptosis; no dying cells were observed.
Specimen 5I: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS8: embryo Present Single cells 5I , 5I'' This protein is a marker of apoptosis; few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
TS8: extraembryonic component Absent 5I'' , 5I
Specimen 5J: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS8: embryo Present Single cells 5J , 5J'' This protein is a marker of apoptosis; few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
TS8: extraembryonic component Absent 5J'' , 5J
Specimen 5K: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS8: embryo Present Single cells 5K , 5K'' This protein is a marker of apoptosis; few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
TS8: extraembryonic component Absent 5K , 5K''
Specimen 5L: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS8: embryo Present Single cells 5L'' , 5L This protein is a marker of apoptosis; few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
TS8: extraembryonic component Absent 5L , 5L''
Specimen 5M: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS8: embryo Present Single cells 5M'' , 5M This protein is a marker of apoptosis; few dying cells were observed. Mutants appear to stall just prior to the onset of gastrulation.
TS8: extraembryonic component Absent 5M , 5M''
Specimen 5N: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Double labeled: white - Casp3; red - Pou5f1 (assay MGI:6445528).
Structure Level Pattern Image Note
TS7: conceptus Absent 5N , 5N'' This protein is a marker of apoptosis; no dying cells were observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory