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Gene Expression Data
Assay Details
Assay
Reference: J:290315 Cheong A, et al., Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development. 2020 May 26;147(10):dev188714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6445528
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (ab27985)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3A: embryonic day 7.5 (more )
Note: Littermate control; embryo is either +/+ or +/-. Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS11: embryo Absent 3A' , 3A
Specimen 3B: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: epiblast Present 3B' , 3B Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 3C: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: epiblast Present 3C , 3C' Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 3D: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: epiblast Present 3D , 3D' Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 3E: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: epiblast Present 3E' , 3E Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 3F: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: epiblast Present 3F , 3F' Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 3G: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Double labeled: red - Pou5f1; green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS7: epiblast Present 3G' , 3G
Specimen 5H: embryonic day 7.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS11: embryo Absent 5H' , 5H
Specimen 5I: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS8: epiblast Present 5I' , 5I Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 5J: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS8: epiblast Present 5J' , 5J Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 5K: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS8: epiblast Present 5K' , 5K Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 5L: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS8: epiblast Present 5L' , 5L Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 5M: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS8: epiblast Present 5M' , 5M Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 5N: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Double labeled: red - Pou5f1; white - Casp3 (assay MGI:6445617).
Structure Level Pattern Image Note
TS7: epiblast Present 5N , 5N'
Specimen 6B: embryonic day 7.5 (more )
Note: Littermate control; this embryo might have been a heterozygote. Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS11: embryo Absent 6B'' , 6B
Specimen 6C: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS8: epiblast Present 6C , 6C'' Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 6D: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS8: epiblast Present 6D'' , 6D Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 6E: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS8: epiblast Present 6E , 6E'' Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 6F: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS8: epiblast Present 6F'' , 6F Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 6G: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS8: epiblast Present 6G , 6G'' Mutants appear to stall just prior to the onset of gastrulation. Thus, the site of expression (the epiblast) is retained in the mutant.
Specimen 6H: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Double labeled: red - Pou5f1; green - mtCO2 (assay MGI:6458461)
Structure Level Pattern Image Note
TS7: epiblast Present 6H , 6H''
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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