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Gene Expression Data
Assay Details
Assay
Reference: J:290315 Cheong A, et al., Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development. 2020 May 26;147(10):dev188714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6458461
Gene symbol: mt-Co2
Gene name: mitochondrially encoded cytochrome c oxidase II
Antibody: anti-mt-CO2 (#55070-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Assay notes: Antibody information provided by author via direct communication.
Results
Specimen 6B: embryonic day 7.5 (more )
Note: Littermate control; embryo is either +/+ or +/-. Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS11: embryo Present 6B' , 6B
Specimen 6C: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS8: epiblast Absent 6C , 6C' Staining is largely absent in null cells.
Specimen 6D: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS8: epiblast Absent 6D , 6D' Staining is largely absent in null cells.
Specimen 6E: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS8: epiblast Absent 6E' , 6E Staining is largely absent in null cells.
Specimen 6F: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS8: epiblast Absent 6F' , 6F Staining is largely absent in null cells.
Specimen 6G: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS8: epiblast Absent 6G , 6G' Staining is largely absent in null cells; the apparent signal observed in the visceral endoderm is the result of secondary antibody binding to endogenous mouse immunoglobulin G.
Specimen 6H: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Double labeled: green - mt-Co2; red - Pou5f1 (assay MGI:6445528)
Structure Level Pattern Image Note
TS7: epiblast Present 6H' , 6H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory