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Gene Expression Data
Assay Details
Assay
Reference: J:290315 Cheong A, et al., Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development. 2020 May 26;147(10):dev188714
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6445603
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachury (N-19; sc-17743)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3A: embryonic day 7.5 (more )
Note: Littermate control; embryo is either +/+ or +/-. Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS11: embryo mesoderm Present 3A''' Expression is seen in the primitive streak and nascent mesoderm.
TS11: primitive streak Present 3A''' Expression is seen in the primitive streak and nascent mesoderm.
Specimen 3B: embryonic day 7.5; Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J (more )
Note: Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 3B''' Mutants appear to stall just prior to the onset of gastrulation.
Specimen 3C: embryonic day 7.5; Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp (more )
Note: Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 3C''' Mutants appear to stall just prior to the onset of gastrulation.
Specimen 3D: embryonic day 7.5; Mrpl44em1(IMPC)J/Mrpl44em1(IMPC)J (more )
Note: Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 3D''' Mutants appear to stall just prior to the onset of gastrulation.
Specimen 3E: embryonic day 7.5; Mrps18ctm1.1(KOMP)Vlcg/Mrps18ctm1.1(KOMP)Vlcg (more )
Note: Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 3E''' Mutants appear to stall just prior to the onset of gastrulation.
Specimen 3F: embryonic day 7.5; Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg (more )
Note: Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS8: conceptus Absent 3F''' Mutants appear to stall just prior to the onset of gastrulation.
Specimen 3G: embryonic day 5.5 (more )
Note: Early egg-cylinder stage embryo. Triple labeled: red - T and Pou5f1 (Oct4; assay MGI:6445528); green - Cdh1 (assay MGI:6445567).
Structure Level Pattern Image Note
TS7: conceptus Absent 3G'''
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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