Sycp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6435158
Gene symbol:	Sycp3
Gene name:	synaptonemal complex protein 3

Antibody:	anti-Sycp3
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1C WT Leptotene ©

Specimen 1C-WT-leptotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C WT Leptotene

1C WT Zygotene ©

Specimen 1C-WT-zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C WT Zygotene

1C WT Pachytene ©

Specimen 1C-WT-pachytene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C WT Pachytene

1C WT Diplotene ©

Specimen 1C-WT-diplotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C WT Diplotene

1C mut Leptotene ©

Specimen 1C-mut-leptotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C mut Leptotene

1C mut Zygotene ©

Specimen 1C-mut-zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C mut Zygotene

1C mut Pachytene ©

Specimen 1C-mut-pachytene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C mut Pachytene

1C mut Diplotene ©

Specimen 1C-mut-diplotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		1C mut Diplotene

S1H upper ©

Specimen S1H-upper: postnatal week 12 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red) and DAPI (blue). The tissue was fixed in either Bouin's or 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S1H upper

S1H lower ©

Specimen S1H-lower: postnatal week 12 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red) and DAPI (blue). The tissue was fixed in either Bouin's or 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S1H lower

S1I upper ©

Specimen S1I-upper: postnatal week 12; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red) and DAPI (blue).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S1I upper

S1I lower ©

Specimen S1I-lower: postnatal week 12; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red) and DAPI (blue).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S1I lower

1E WT Leptotene ©

Specimen 1E-WT-leptotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E WT Leptotene

1E WT Zygotene ©

Specimen 1E-WT-zygotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E WT Zygotene

1E WT Pachytene ©

Specimen 1E-WT-pachytene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red). The tissue was fixed in either Bouin's or 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E WT Pachytene

1E WT Diplotene ©

Specimen 1E-WT-diplotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red). The tissue was fixed in either Bouin's or 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E WT Diplotene

1E mut Leptotene ©

Specimen 1E-mut-leptotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E mut Leptotene

1E mut Zygotene ©

Specimen 1E-mut-zygotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E mut Zygotene

1E mut Pachytene ©

Specimen 1E-mut-pachytene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E mut Pachytene

1E mut Diplotene ©

Specimen 1E-mut-diplotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Also stained for H3K4me3 (red).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		1E mut Diplotene

4A WT Leptotene ©

Specimen 4A-WT-leptotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A WT Leptotene

4A WT Zygotene ©

Specimen 4A-WT-zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A WT Zygotene

4A WT Pachytene ©

Specimen 4A-WT-pachytene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A WT Pachytene

4A WT Diplotene ©

Specimen 4A-WT-diplotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A WT Diplotene

4A mut Leptotene ©

Specimen 4A-mut-leptotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A mut Leptotene

4A mut Zygotene ©

Specimen 4A-mut-zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A mut Zygotene

4A mut Pachytene ©

Specimen 4A-mut-pachytene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A mut Pachytene

4A mut Diplotene ©

Specimen 4A-mut-diplotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A mut Diplotene

4C WT EZ ©

Specimen 4C-WT-early zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C WT EZ

4C WT LZ ©

Specimen 4C-WT-late zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C WT LZ

4C WT EP ©

Specimen 4C-WT-early pachytene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C WT EP

4C mut EZ ©

Specimen 4C-mut-early zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C mut EZ

4C mut LZ ©

Specimen 4C-mut-late zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C mut LZ

4C mut EP ©

Specimen 4C-mut-early pachytene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4C mut EP

4D WT EZ ©

Specimen 4D-WT-early zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D WT EZ

4D WT LZ ©

Specimen 4D-WT-late zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D WT LZ

4D WT EP ©

Specimen 4D-WT-early pachytene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D WT EP

4D mut EZ ©

Specimen 4D-mut-early zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D mut EZ

4D mut LZ ©

Specimen 4D-mut-late zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D mut LZ

4D mut EP ©

Specimen 4D-mut-early pachytene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D mut EP

5C WT ©

Specimen 5C-WT-diplotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C WT

5C mut ©

Specimen 5C-mut-diplotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5C mut	An increase in precocious separation of homologous chromosomes is observed in the mutants.

5E WT ©

Specimen 5E-WT-pachytene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Triple labeled: green - Sycp3; blue - Ss18l1 (assay MGI:6435219); red - Mlh1 (assay MGI:6435218)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5E WT

5E mut ©

Specimen 5E-mut-pachytene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Sycp3; blue - Ss18l1 (assay MGI:6435219); red - Mlh1 (assay MGI:6435218)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		5E mut

8F WT ©

Specimen 8F-WT: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Mlh1 (assay MGI:6435218)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8F WT	Expression is seen in the synaptonemal complex.

8F mut ©

Specimen 8F-mut: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Mlh1 (assay MGI:6435218)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8F mut	Expression is seen in the synaptonemal complex; it forms in the mutants.

Specimen 8H-WT-leptotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Leptotene

Specimen 8H-WT-zygotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Zygotene

Specimen 8H-WT-pachytene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Pachytene

Specimen 8H-WT-diplotene: embryonic day 17.5 (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H WT Diplotene

Specimen 8H-mut-leptotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Leptotene

Specimen 8H-mut-zygotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Zygotene

Specimen 8H-mut-pachytene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Pachytene

Specimen 8H-mut-diplotene: embryonic day 17.5; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present		8H mut Diplotene	Precocious separation of homologous chromosomes was observed in the mutant. This is quantified in Fig. 8I.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24