Cxxc1^tm1.2Liwa
Targeted Allele Detail

Summary

• Symbol: Cxxc1^tm1.2Liwa
• Name: CXXC finger protein 1; targeted mutation 1.2, Lie Wang
• MGI ID: MGI:5882523
• Gene: Cxxc1 Location: Chr18:74349283-74354564 bp, + strand Genetic Position: Chr18, 50.7 cM
• Alliance: Cxxc1^tm1.2Liwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239921
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 5. Subsequently, the neo cassette was removed through flp-mediated recombination and exons 4 and 5 were deleted through cre-mediated recombination, leaving a null allele. RT-qPCR confirmed the absence or significant reduction in transcription from this allele. Western blots confirmed virtual absence of peptides translated from this allele. (J:239921)

Expression

In Mice Carrying this Mutation:

55 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cxxc1 Mutation: 28 strains or lines available

References

• Original: J:239921 Cao W, et al., CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation. Nat Commun. 2016 May 23;7:11687
• All: 1 reference(s)