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Gene Expression Data
Assay Details
Assay
Reference: J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6435205
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phospho-histone H2A.X (20E3) (CST;#9718)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4A-WT-leptotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Leptotene Staining is seen throughout the nucleus.
Specimen 4A-WT-zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Zygotene Staining is seen throughout the nucleus.
Specimen 4A-WT-pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Pachytene Staining is largely restricted to the sex body.
Specimen 4A-WT-diplotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Diplotene Staining is restricted to the sex body.
Specimen 4A-mut-leptotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Leptotene As in wild type, staining is seen throughout the nucleus.
Specimen 4A-mut-zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Zygotene As in wild type, staining is seen throughout the nucleus.
Specimen 4A-mut-pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Pachytene As in wild type, staining is largely restricted to the sex body. However, the number of autosomes with unrepaired double stranded breaks is significantly increased in the mutant.
Specimen 4A-mut-diplotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - H2AX; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Diplotene Staining is seen on the sex body.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory