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Gene Expression Data
Assay Details
Assay
Reference: J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6435158
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: anti-Sycp3
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1C-WT-leptotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C WT Leptotene
Specimen 1C-WT-zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C WT Zygotene
Specimen 1C-WT-pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C WT Pachytene
Specimen 1C-WT-diplotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C WT Diplotene
Specimen 1C-mut-leptotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C mut Leptotene
Specimen 1C-mut-zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C mut Zygotene
Specimen 1C-mut-pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C mut Pachytene
Specimen 1C-mut-diplotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS28: spermatocyte Present 1C mut Diplotene
Specimen S1H-upper: postnatal week 12 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red) and DAPI (blue). The tissue was fixed in either Bouin's or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: spermatocyte Present S1H upper
Specimen S1H-lower: postnatal week 12 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red) and DAPI (blue). The tissue was fixed in either Bouin's or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: spermatocyte Present S1H lower
Specimen S1I-upper: postnatal week 12; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red) and DAPI (blue).
Structure Level Pattern Image Note
TS28: spermatocyte Present S1I upper
Specimen S1I-lower: postnatal week 12; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red) and DAPI (blue).
Structure Level Pattern Image Note
TS28: spermatocyte Present S1I lower
Specimen 1E-WT-leptotene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS25: oocyte Present 1E WT Leptotene
Specimen 1E-WT-zygotene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS25: oocyte Present 1E WT Zygotene
Specimen 1E-WT-pachytene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red). The tissue was fixed in either Bouin's or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: oocyte Present 1E WT Pachytene
Specimen 1E-WT-diplotene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Also stained for H3K4me3 (red). The tissue was fixed in either Bouin's or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: oocyte Present 1E WT Diplotene
Specimen 1E-mut-leptotene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS25: oocyte Present 1E mut Leptotene
Specimen 1E-mut-zygotene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS25: oocyte Present 1E mut Zygotene
Specimen 1E-mut-pachytene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS25: oocyte Present 1E mut Pachytene
Specimen 1E-mut-diplotene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Also stained for H3K4me3 (red).
Structure Level Pattern Image Note
TS25: oocyte Present 1E mut Diplotene
Specimen 4A-WT-leptotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Leptotene
Specimen 4A-WT-zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Zygotene
Specimen 4A-WT-pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Pachytene
Specimen 4A-WT-diplotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A WT Diplotene
Specimen 4A-mut-leptotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Leptotene
Specimen 4A-mut-zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Zygotene
Specimen 4A-mut-pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Pachytene
Specimen 4A-mut-diplotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - H2AX (assay MGI:6435205)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A mut Diplotene
Specimen 4C-WT-early zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C WT EZ
Specimen 4C-WT-late zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C WT LZ
Specimen 4C-WT-early pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C WT EP
Specimen 4C-mut-early zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C mut EZ
Specimen 4C-mut-late zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C mut LZ
Specimen 4C-mut-early pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Dmc1 (assay MGI:6435212)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C mut EP
Specimen 4D-WT-early zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D WT EZ
Specimen 4D-WT-late zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D WT LZ
Specimen 4D-WT-early pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D WT EP
Specimen 4D-mut-early zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D mut EZ
Specimen 4D-mut-late zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D mut LZ
Specimen 4D-mut-early pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Rad51 (assay MGI:6435213)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D mut EP
Specimen 5C-WT-diplotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C WT
Specimen 5C-mut-diplotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS28: spermatocyte Present 5C mut An increase in precocious separation of homologous chromosomes is observed in the mutants.
Specimen 5E-WT-pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Triple labeled: green - Sycp3; blue - Ss18l1 (assay MGI:6435219); red - Mlh1 (assay MGI:6435218)
Structure Level Pattern Image Note
TS28: spermatocyte Present 5E WT
Specimen 5E-mut-pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Triple labeled: green - Sycp3; blue - Ss18l1 (assay MGI:6435219); red - Mlh1 (assay MGI:6435218)
Structure Level Pattern Image Note
TS28: spermatocyte Present 5E mut
Specimen 8F-WT: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Mlh1 (assay MGI:6435218)
Structure Level Pattern Image Note
TS25: oocyte Present 8F WT Expression is seen in the synaptonemal complex.
Specimen 8F-mut: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Mlh1 (assay MGI:6435218)
Structure Level Pattern Image Note
TS25: oocyte Present 8F mut Expression is seen in the synaptonemal complex; it forms in the mutants.
Specimen 8H-WT-leptotene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H WT Leptotene
Specimen 8H-WT-zygotene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H WT Zygotene
Specimen 8H-WT-pachytene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H WT Pachytene
Specimen 8H-WT-diplotene: embryonic day 17.5 (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H WT Diplotene
Specimen 8H-mut-leptotene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H mut Leptotene
Specimen 8H-mut-zygotene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H mut Zygotene
Specimen 8H-mut-pachytene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H mut Pachytene
Specimen 8H-mut-diplotene: embryonic day 17.5; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: green - Sycp3; red - Sycp1 (assay MGI:6435217)
Structure Level Pattern Image Note
TS25: oocyte Present 8H mut Diplotene Precocious separation of homologous chromosomes was observed in the mutant. This is quantified in Fig. 8I.
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05/07/2024
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