Rad51 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6435213
Gene symbol:	Rad51
Gene name:	RAD51 recombinase

Antibody:	anti-Rad51 (ab176458)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

4D WT EZ ©

Specimen 4D-WT-early zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Rad51; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D WT EZ	Expression is present in foci.

4D WT LZ ©

Specimen 4D-WT-late zygotene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Rad51; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D WT LZ	Expression is present in foci.

4D WT EP ©

Specimen 4D-WT-early pachytene: postnatal adult (more

)
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Rad51; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D WT EP	Expression is present in foci.

4D mut EZ ©

Specimen 4D-mut-early zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Rad51; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D mut EZ	Expression is present in foci; the number of these foci in the mutant is comparable to the number in wild type.

4D mut LZ ©

Specimen 4D-mut-late zygotene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Rad51; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D mut LZ	Expression is present in foci; the number of these foci in the mutant is comparable to the number in wild type.

4D mut EP ©

Specimen 4D-mut-early pachytene: postnatal adult; Cxxc1^tm1.1Liwa/Cxxc1^tm1.2Liwa, Stra8^{em1(GFP/cre)Smoc}/Stra8⁺ (more

)
Note: Conditional mutant. Double labeled: red - Rad51; green - Sycp3 (assay MGI:6435158)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4D mut EP	Expression is present in foci; the number of these foci in the mutant is increased compared to the number in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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