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Gene Expression Data
Assay Details
Assay
Reference: J:268273 Simon CS, et al., A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open. 2018 Dec 10;7(12):bio036517
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6434288
Gene symbol: Gata4
Gene name: GATA binding protein 4
Reporter: GFP
Direct Detection
Results
Specimen 2A E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (61 cells). Double labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2A E3.5 Merge , 2A E3.5 Merge DNA , 2A E3.5 GFP
Specimen 2A E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (76 cells). Double labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A E3.75 GFP , 2A E3.75 Merge DNA , 2A E3.75 Merge
Specimen 2A E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (110 cells). Double labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A E4.0 Merge , 2A E4.0 GFP , 2A E4.0 Merge DNA
Specimen 2A E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (141 cells). Double labeled: green - Gata4 reporter; blue- Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2A E4.5 GFP , 2A E4.5 Merge , 2A E4.5 Merge DNA
Specimen 2C E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (61 cells). Triple labeled: green - Gata4 reporter; blue - Gata6 (assay MGI:6434291), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2C E3.5 MergeDNA , 2C E3.5 Merge , 2C E3.5 GFP
Specimen 2C E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (81 cells). Triple labeled: green - Gata4 reporter; blue - Gata6 (assay MGI:6434291), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2C E3.75 GFP , 2C E3.75 Merge , 2C E3.75 MergeDNA
Specimen 2C E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (106 cells). Triple labeled: green - Gata4 reporter; blue - Gata6 (assay MGI:6434291), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2C E4.0 GFP , 2C E4.0 MergeDNA , 2C E4.0 Merge
Specimen 2C E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (133 cells). Triple labeled: green - Gata4 reporter; blue - Gata6 (assay MGI:6434291), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS6: blastocyst Present 2C E4.5 Merge , 2C E4.5 MergeDNA , 2C E4.5 GFP
Specimen 3A top: embryonic day 5.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3A GFP/GATA4 top , 3A MergeDNA top , 3A GFP top
TS8: primitive endoderm Present 3A GFP/GATA4 top , 3A MergeDNA top , 3A GFP top
TS8: epiblast Absent 3A GFP/GATA4 top , 3A MergeDNA top , 3A GFP top
TS8: extraembryonic ectoderm Absent 3A GFP/GATA4 top , 3A MergeDNA top , 3A GFP top
Specimen 3A bottom: embryonic day 5.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3A GFP/GATA4 bottom , 3A MergeDNA bottom , 3A GFP bottom
TS8: primitive endoderm Present 3A GFP/GATA4 bottom , 3A MergeDNA bottom , 3A GFP bottom
TS8: epiblast Absent
TS8: extraembryonic ectoderm Absent
Specimen 3B top: embryonic day 6.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Pre-streak embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3B GFP top , 3B MergeDNA top , 3B GFP/GATA4 top
TS8: primitive endoderm Present 3B GFP top , 3B MergeDNA top , 3B GFP/GATA4 top
TS8: epiblast Absent 3B GFP top , 3B GFP/GATA4 top
TS8: extraembryonic ectoderm Absent 3B GFP top , 3B GFP/GATA4 top
Specimen 3B bottom: embryonic day 6.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Pre-streak embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 3B GFP/GATA4 bottom , 3B MergeDNA bottom , 3B GFP bottom
TS8: primitive endoderm Present 3B GFP/GATA4 bottom , 3B MergeDNA bottom , 3B GFP bottom
TS8: epiblast Absent 3B GFP bottom , 3B GFP/GATA4 bottom , 3B MergeDNA bottom
TS8: extraembryonic ectoderm Absent 3B GFP bottom , 3B GFP/GATA4 bottom , 3B MergeDNA bottom
Specimen 4A top: embryonic day 6.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-streak stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 4A GFP top , 4A Merge top , 4A GFP/GATA4 top
TS9: primitive endoderm Present 4A GFP top , 4A Merge top , 4A GFP/GATA4 top
Specimen 4A bottom: embryonic day 6.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-streak stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 4A GFP bottom , 4A Merge bottom , 4A GFP/GATA4 bottom
TS9: primitive endoderm Present 4A GFP bottom , 4A Merge bottom , 4A GFP/GATA4 bottom
Specimen 4B top: embryonic day 7.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early bud stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS11: primitive endoderm Present Regionally restricted 4B GFP top , 4B Merge top , 4B GFP/GATA4 top Expression is sparse overlying the epiblast.
TS11: embryo mesoderm Present 4B Merge top , 4B GFP top , 4B GFP/GATA4 top
Specimen 4B bottom: embryonic day 7.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early bud stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS11: primitive endoderm Present Regionally restricted 4B GFP bottom , 4B GFP/GATA4 bottom , 4B Merge bottom Expression is sparse overlying the epiblast.
TS11: embryo mesoderm Present 4B GFP/GATA4 bottom , 4B Merge bottom , 4B GFP bottom
Specimen 4C top: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late streak stage embryo. Double labeled: green - Gata4 reporter; red - T (assay MGI:6434314).
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Strong 4C Merge top , 4C GFP top
TS11: extraembryonic mesoderm Weak 4C GFP top , 4C Merge top
Specimen 4C bottom: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late streak stage embryo. Double labeled: green - Gata4 reporter; red - T (assay MGI:6434314).
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Strong 4C Merge bottom , 4C c GFP , 4C c Merge , 4C GFP bottom
TS11: extraembryonic mesoderm Weak 4C GFP bottom , 4C c Merge , 4C c GFP , 4C Merge bottom
TS11: primitive endoderm Present 4C d GFP , 4C d Merge , 4C GFP bottom , 4C Merge bottom
Specimen 4Ca: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late streak stage embryo. Double labeled: green - Gata4 reporter; red - T (assay MGI:6434314).
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Strong 4C a GFP , 4C a Merge
TS11: extraembryonic mesoderm Weak 4C a Merge , 4C a GFP
Specimen 4Cb: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late streak stage embryo. Double labeled: green - Gata4 reporter; red - T (assay MGI:6434314).
Structure Level Pattern Image Note
TS11: extraembryonic mesoderm Weak 4C b GFP , 4C b Merge
TS11: lateral plate mesoderm Weak 4C b GFP , 4C b Merge
TS11: primitive endoderm Weak 4C b GFP , 4C b Merge
TS11: splanchnopleure Weak 4C b GFP , 4C b Merge
Specimen 5 left: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS13: heart Present 5_left top , 5_left bottom
Specimen 5a: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS13: primitive ventricle Present Regionally restricted 5a MergeDNA , 5a GFP , 5a GFP/GATA4 Expression is in both the outer myocardium and the inner endocardium.
TS13: primitive ventricle cardiac muscle Present 5a GFP/GATA4 , 5a GFP , 5a MergeDNA
Specimen 5b: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS13: primitive ventricle Present Regionally restricted 5b GFP , 5b MergeDNA , 5b GFP/GATA4 Expression is in both the outer myocardium and the inner endocardium.
TS13: primitive ventricle cardiac muscle Present 5b MergeDNA , 5b GFP , 5b GFP/GATA4
TS13: septum transversum Present 5b MergeDNA , 5b GFP , 5b GFP/GATA4
TS13: sinus venosus Present 5b MergeDNA , 5b GFP , 5b GFP/GATA4
TS13: midgut endoderm Present Regionally restricted 5b MergeDNA Expression is in the anterior midgut.
TS13: yolk sac endoderm Present 5b MergeDNA
TS13: foregut diverticulum endoderm Absent 5b MergeDNA
TS13: hindgut diverticulum endoderm Absent 5b MergeDNA
Specimen 6A: embryonic day 9.5; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS15: heart Present 6A top right , 6A top left
TS15: midgut epithelium Present 6A top right , 6A top left
TS15: septum transversum Present 6A top right , 6A top left
Specimen 6Aa: embryonic day 9.5; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS15: heart Present 6A a right , 6A a left
Specimen 6Ab: embryonic day 9.5; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS15: septum transversum Present 6A b right , 6A b left
TS15: foregut Absent 6A b right , 6A b left
Specimen 6Ac: embryonic day 9.5; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS15: midgut epithelium Strong 6A c left , 6A c right
TS15: foregut Absent 6A c right , 6A c left
TS15: hindgut Absent 6A c right , 6A c left
Specimen 6B: embryonic day 12.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Fixative was 1% paraformaldehyde.
Structure Level Pattern Image Note
TS20: cardiac muscle tissue Present 6B right , 6B left
TS20: endocardial lining Present 6B right , 6B left
TS20: liver Present 6B right , 6B left
TS20: midgut epithelium Present 6B right , 6B left
TS20: midgut mesenchyme Present 6B right , 6B left
TS20: pancreas Present 6B right , 6B left
TS20: stomach epithelium Present 6B right , 6B left
TS20: stomach mesenchyme Present 6B right , 6B left
TS20: lung epithelium Absent 6B right , 6B left
Specimen 6C top left: embryonic day 12.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Fixative was 1% paraformaldehyde.
Structure Level Pattern Image Note
TS20: stomach epithelium Present 6C top left
TS20: stomach mesenchyme Present 6C top left
Specimen 6C top right: embryonic day 12.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Fixative was 1% paraformaldehyde.
Structure Level Pattern Image Note
TS20: cardiac muscle tissue Strong 6C top right
TS20: endocardial lining Strong 6C top right
TS20: liver Present Regionally restricted 6C top right Expression is in the mesenchyme.
Specimen 6C bottom left: embryonic day 12.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Fixative was 1% paraformaldehyde.
Structure Level Pattern Image Note
TS20: midgut epithelium Present 6C bottom left
TS20: pancreas Present 6C bottom left
TS20: midgut mesenchyme Present Regionally restricted 6C bottom left Expression is in the epithelium associated mesenchyme.
Specimen 6C bottom right: embryonic day 12.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Fixative was 1% paraformaldehyde.
Structure Level Pattern Image Note
TS20: liver Present Regionally restricted 6C bottom right Expression is in the mesenchyme.
TS20: lung mesenchyme Present 6C bottom right
TS20: lung epithelium Absent 6C bottom right
Specimen 7 Ovary: postnatal month 3; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS28: ovary Present 7_Ovary
Specimen 7 Testis: postnatal month 3; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS28: testis Present 7_Testis
Specimen 7 Heart: postnatal month 3; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS28: heart Present 7_Heart
Specimen 7 Lung: postnatal month 3; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS28: lung Present 7_Lung
Specimen 7 Adrenal Gland: postnatal month 3; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS28: adrenal gland Present 7_Adrenal Gland
Specimen 7 Liver: postnatal month 3; Gata4tm1.1Hadj/Gata4+ (more )
Structure Level Pattern Image Note
TS28: gallbladder Present 7_Liver
Specimen S1D E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (75 cells). Triple labeled: green - Gata4 reporter, blue - Sox17 (assay MGI:6434516), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present S1D E3.75 GFP , S1D E3.75 Merge DNA , S1D E3.75 Merge
Specimen S1D E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (148 cells). Triple labeled: green - Gata4 reporter, blue - Sox17 (assay MGI:6434516), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS6: blastocyst Present S1D E4.5 Merge , S1D E4.5 GFP , S1D E4.5 MergeDNA
Specimen S1E: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (153 cells). Triple labeled: green - Gata4 reporter, blue - Sox7 (assay MGI:6434523), red - Nanog (assay MGI:434292).
Structure Level Pattern Image Note
TS6: blastocyst Present S1E MergeDNA , S1E Merge , S1E GFP
Specimen S3A top: embryonic day 6.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early streak stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present S3A GFP/GATA4 top , S3A MergeDNA top , S3A GFP top
TS9: primitive endoderm Present S3A GFP/GATA4 top , S3A MergeDNA top , S3A GFP top
Specimen S3A bottom: embryonic day 6.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early streak stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present S3A MergeDNA bottom , S3A GFP/GATA4 bottom , S3A GFP bottom
TS9: primitive endoderm Present S3A MergeDNA bottom , S3A GFP/GATA4 bottom , S3A GFP bottom
Specimen S3B top: embryonic day 7.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early head fold stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS12: primitive endoderm Present Regionally restricted S3B GFP/GATA4 top , S3B MergeDNA top , S3B GFP top Expression is sparse overlying the epiblast.
TS12: embryo mesoderm Present Regionally restricted S3B GFP/GATA4 top , S3B MergeDNA top , S3B GFP top Expression is in the mesoderm layer at the proximal-anterior region of the embryo.
Specimen S3B bottom: embryonic day 7.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early head fold stage embryo. Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS12: primitive endoderm Present Regionally restricted S3B MergeDNA bottom , S3B GFP/GATA4 bottom , S3B GFP bottom Expression is sparse overlying the epiblast.
TS12: embryo mesoderm Present Regionally restricted S3B MergeDNA bottom , S3B GFP bottom , S3B GFP/GATA4 bottom Expression is in the mesoderm layer at the proximal-anterior region of the embryo.
Specimen S4 left: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS13: midgut Present S4_left panel
TS13: yolk sac Present S4_left panel
Specimen S4 right: embryonic day 8.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Double labeled: green - Gata4 reporter; red - Gata4 protein (assay MGI:6434233).
Structure Level Pattern Image Note
TS13: yolk sac Present S4_GFP/GATA4 , S4_MergeDNA , S4_GFP
TS13: midgut Present Regionally restricted S4_GFP , S4_MergeDNA , S4_GFP/GATA4 Expression is in the anterior midgut and is absent in the posterior midgut.
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05/21/2024
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