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Gene Expression Data
Assay Details
Assay
Reference: J:268273 Simon CS, et al., A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open. 2018 Dec 10;7(12):bio036517
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6434314
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachyury (AF2085)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4C top: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS11: primitive streak Present 4C T top , 4C Merge top
TS11: embryo mesoderm Weak 4C Merge top , 4C T top
Specimen 4C bottom: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS11: primitive streak Present 4C T bottom , 4C d T , 4C d Merge , 4C Merge bottom
TS11: embryo mesoderm Weak 4C c Merge , 4C c T , 4C Merge bottom , 4C T bottom
Specimen 4Ca: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS11: primitive streak Present 4C a Merge , 4C a T
TS11: embryo mesoderm Present Regionally restricted 4C a Merge , 4C a T Expression is posterior.
Specimen 4Cb: embryonic day 7.25; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late-streak stage embryo. Double labeled: red - T; green - Gata4 reporter (assay MGI:6434288).
Structure Level Pattern Image Note
TS11: primitive streak Present 4C b T , 4C b Merge
TS11: embryo mesoderm Present Regionally restricted 4C b T , 4C b Merge Expression is posterior.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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