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Gene Expression Data
Assay Details
Assay
Reference: J:268273 Simon CS, et al., A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open. 2018 Dec 10;7(12):bio036517
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6434291
Gene symbol: Gata6
Gene name: GATA binding protein 6
Antibody: anti-Gata6 (AF1700)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2C E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (61 cells). Triple labeled: blue - Gata6; green - Gata4 reporter (assay MGI:6434288); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2C E3.5 GATA6 , 2C E3.5 MergeDNA , 2C E3.5 Merge

Specimen 2C E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (81 cells). Triple labeled: blue - Gata6; green - Gata4 reporter (assay MGI:6434288); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2C E3.75 MergeDNA , 2C E3.75 GATA6 , 2C E3.75 Merge

Specimen 2C E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (106 cells). Triple labeled: blue - Gata6; green - Gata4 reporter (assay MGI:6434288); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2C E4.0 MergeDNA , 2C E4.0 GATA6 , 2C E4.0 Merge

Specimen 2C E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (133 cells). Triple labeled: blue - Gata6; green - Gata4 reporter (assay MGI:6434288); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2C E4.5 GATA6 , 2C E4.5 Merge , 2C E4.5 MergeDNA


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory