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Gene Expression Data
Assay Details
Assay
Reference: J:268273 Simon CS, et al., A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open. 2018 Dec 10;7(12):bio036517
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6434292
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog (RCAB0002P-F/ RCAB001P)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2C E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (61 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Gata6 (assay MGI:6434291).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2C E3.5 MergeDNA , 2C E3.5 NANOG , 2C E3.5 Merge
Specimen 2C E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (81 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Gata6 (assay MGI:6434291).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2C E3.75 NANOG , 2C E3.75 MergeDNA , 2C E3.75 Merge
Specimen 2C E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (106 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Gata6 (assay MGI:6434291).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2C E4.0 Merge , 2C E4.0 NANOG , 2C E4.0 MergeDNA
Specimen 2C E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (133 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Gata6 (assay MGI:6434291).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2C E4.5 MergeDNA , 2C E4.5 NANOG , 2C E4.5 Merge
Specimen S1B E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (62 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434513); blue - Gata4 protein (assay MGI:6434506).
Structure Level Pattern Image Note
TS4: inner cell mass Present S1B E3.5 Merge DNA , S1B E3.5 NANOG , S1B E3.5 Merge
Specimen S1B E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (81 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434513); blue - Gata4 protein (assay MGI:6434506).
Structure Level Pattern Image Note
TS5: inner cell mass Present S1B E3.75 Merge , S1B E3.75 Merge DNA , S1B E3.75 NANOG
Specimen S1B E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (115 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434513); blue - Gata4 protein (assay MGI:6434506).
Structure Level Pattern Image Note
TS5: inner cell mass Present S1B E4.0 NANOG , S1B E4.0 Merge DNA , S1B E4.0 Merge
Specimen S1B E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (161 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434513); blue - Gata4 protein (assay MGI:6434506).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S1B E4.5 NANOG , S1B E4.5 Merge DNA , S1B E4.5 Merge
Specimen S1D E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (75 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Sox17 (assay MGI:6434516).
Structure Level Pattern Image Note
TS5: inner cell mass Present S1D E3.75 Merge , S1D E3.75 Merge DNA , S1D E3.75 NANOG
Specimen S1D E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (148 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Sox17 (assay MGI:6434516).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S1D E4.5 NANOG , S1D E4.5 Merge , S1D E4.5 MergeDNA
Specimen S1E: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (153 cells). Triple labeled: red - Nanog; green - Gata4 reporter (assay MGI:6434288); blue - Sox7 (assay MGI:6434523).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S1E NANOG , S1E Merge , S1E MergeDNA
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory