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Gene Expression Data
Assay Details
Assay
Reference: J:268273 Simon CS, et al., A Gata4 nuclear GFP transcriptional reporter to study endoderm and cardiac development in the mouse. Biol Open. 2018 Dec 10;7(12):bio036517
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6434513
Gene symbol: Gata4
Gene name: GATA binding protein 4
Reporter: GFP
Antibody: anti-GFP
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S1B E3.5: embryonic day 3.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Early stage blastocyst (62 cells). Triple labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434506); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS4: inner cell mass Present S1B E3.5 GFP , S1B E3.5 Merge DNA , S1B E3.5 Merge
Specimen S1B E3.75: embryonic day 3.75; Gata4tm1.1Hadj/Gata4+ (more )
Note: Mid-stage blastocyst (81 cells). Triple labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434506); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present S1B E3.75 GFP , S1B E3.75 Merge , S1B E3.75 Merge DNA
Specimen S1B E4.0: embryonic day 4.0; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (115 cells). Triple labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434506); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS5: inner cell mass Present S1B E4.0 GFP , S1B E4.0 Merge DNA , S1B E4.0 Merge
Specimen S1B E4.5: embryonic day 4.5; Gata4tm1.1Hadj/Gata4+ (more )
Note: Late stage blastocyst (161 cells). Triple labeled: green - Gata4 reporter; blue - Gata4 protein (assay MGI:6434506); red - Nanog (assay MGI:6434292).
Structure Level Pattern Image Note
TS6: blastocyst Present S1B E4.5 GFP , S1B E4.5 Merge DNA , S1B E4.5 Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory