Calb2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:271730 Kask K, et al., Targeted deletion of RIC8A in mouse neural precursor cells interferes with the development of the brain, eyes, and muscles. Dev Neurobiol. 2018 Apr;78(4):374-390
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6416424
Gene symbol:	Calb2
Gene name:	calbindin 2

Antibody:	anti-Calretinin (sc-11644)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen 2A: embryonic day 14.5 (more

)
Note: Double labeled: green - Calb2; red - Ric8a (assay MGI:6416420). Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2B , 2A	Expression is in Cajal-Retzius cells.

Specimen 2A': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: green - Calb2; red - Ric8a (assay MGI:6416420).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2B' , 2A'	Expression in this mutant is the same as in controls, however, cells were mispositioned in the areas of cortical ectopias and depending on their position were also mishapen.

Specimen 2C: embryonic day 14.5 (more

)
Note: Double labeled: green - Calb2; red - Nes (assay MGI:6416425). Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2D , 2C	Expression is in Cajal-Retzius cells.

Specimen 2C': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: green - Calb2; red - Nes (assay MGI:6416425).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2C' , 2D'	Expression in this mutant is the same as in controls, however, cells were mispositioned in the areas of cortical ectopias and depending on their position were also mishapen.

Specimen 2E: embryonic day 14.5 (more

)
Note: Double labeled: green - Calb2; red - Laminin. Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2F , 2E	Expression is in Cajal-Retzius cells.

Specimen 2E': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: green - Calb2; red - Laminin.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2F' , 2E'	Expression in this mutant is the same as in controls, however, cells were mispositioned in the areas of cortical ectopias and depending on their position were also mishapen.

Specimen not shown: embryonic day 12.5 (more

)
Note: Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted		Expression is in Cajal-Retzius cells.

Specimen not shown cKO: embryonic day 12.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted		Expression in this mutant is the same as in controls.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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