Nes Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:271730 Kask K, et al., Targeted deletion of RIC8A in mouse neural precursor cells interferes with the development of the brain, eyes, and muscles. Dev Neurobiol. 2018 Apr;78(4):374-390
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6416425
Gene symbol:	Nes
Gene name:	nestin

Antibody:	anti-Nestin (2Q178, sc-58813)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen 2C: embryonic day 14.5 (more

)
Note: Double labeled: red - Nes; green - Calb2 (assay MGI:6416424). Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2C , 2D	Expression is in radial glial fibers.

Specimen 2C': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: red - Nes; green - Calb2 (assay MGI:6416424).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2C' , 2D'	In this mutant the radial glial processes were irregular with frequent bundles at the basement membrane breaches.

Specimen 2G: embryonic day 13.5 (more

)
Note: Double labeled: red - Nes; green - laminin. Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	2H , 2G	Expression is in radial glial fibers.

Specimen 2G': embryonic day 13.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: red - Nes; green - laminin.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	2H' , 2G'	In this mutant the radial glial processes were irregular with frequent bundles at the basement membrane breaches.

Specimen S3A: embryonic day 14.5 (more

)
Note: Double labeled: red - Nes; green - laminin. Littermate control; the genotype was either NestinCre+/-Ric8a+/^tm2.1Aks, NestinCre-/-;Ric8a^tm1Aks/^tm2.1Aks, or NestinCre-/-; Ric8a+/^tm2.1Aks.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S3C , S3D , S3A	Expression is in radial glial fibers.

Specimen S3A': embryonic day 14.5; Ric8a^tm1Aks/Ric8a^tm2.1Aks, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Double labeled: red - Nes; green - laminin.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	S3A' , S3C' , S3D'	In this mutant the radial glial processes were irregular with frequent bundles at the basement membrane breaches.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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