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Gene Expression Data
Assay Details
Assay
Reference: J:108721 Nishimura W, et al., A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells. Dev Biol. 2006 May 15;293(2):526-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361003
Gene symbol: Mafb
Gene name: MAF bZIP transcription factor B
Antibody: anti-MafB (BL658)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
Results
Specimen 1E: postnatal adult (more )
Note: Double labeled: green - Mafb; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1E Expression in a few rare beta cells.
Specimen 1F: postnatal adult (more )
Note: Double labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1F Expression in glucagon-expressing alpha cells. Expression
Specimen 4C: embryonic day 10.5 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS17: pancreas primordium Present Regionally restricted 4C Majority (but not all) of Mafb-expressing cells also express glucagon.
Specimen 4F: embryonic day 11.5 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS19: pancreas primordium Present Regionally restricted 4F Majority (but not all) of Mafb-expressing cells also express glucagon.
Specimen 4K: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 4Ka , 4Kb Significant increase in Mafb-expressing cells also expressing glucagon
Specimen 4L: postnatal day 5 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 4L Expression restricted to glucagon-expressing alpha cells.
Specimen 4M: postnatal day 8 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 4M Expression restricted to glucagon-expressing alpha cells.
Specimen 4N: postnatal day 14 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 4N Expression restricted to glucagon-expressing alpha cells.
Specimen 5A: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - synaptophysin (Syp; assay MGI:6361019); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 5A All Mafb-expressing cells also express synaptophysin.
Specimen 5C: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 5C A significant proportion of Mafb-expressing cells also express insulin.
Specimen 6B: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 6B
Specimen 7A: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 7A Fewer Mafb-expressing cells than cell expressing Nkx6-1.
Specimen 7D: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 7D
Specimen 8C: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - Glut2 (Slc2a2; assay MGI:6361021); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 8C
Specimen 8E: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - Glut2 (Slc2a2; assay MGI:6361021); blue - Ins1 (assay MGI:6361014) and Ins2 (assay MGI:6361015).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 8E
Specimen 9C: embryonic day 15.5 (more )
Note: Double labeled: green - Mafb; red - Ki-67 (Mki67; assay MGI:6361022).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 9C
Specimen 9G: postnatal day 14 (more )
Note: Double labeled: green - Mafb; red - Ki-67 (Mki67; assay MGI:6361022).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9G
Specimen 9I: postnatal day 14 (more )
Note: Triple labeled: green - Mafb; red - Ki-67 (Mki67; assay MGI:6361022); blue - Ins1 (assay MGI:6361014) and Ins2 (assay MGI:6361015).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9I Expression in Ki-67 (Mki67)-expressing cell.
Specimen 11D: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafb; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 11D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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