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Gene Expression Data
Assay Details
Assay
Reference: J:108721 Nishimura W, et al., A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells. Dev Biol. 2006 May 15;293(2):526-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361022
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki67
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Texas Red
Results
Specimen 9A: embryonic day 15.5 (more )
Note: Double labeled: red - Ki-67 (Mki67); green - Nkx6-1 (assay MGI:6361020).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 9A Expression in 25% of Nkx6-1-expressing cells.
Specimen 9B: embryonic day 15.5 (more )
Note: Double labeled: red - Ki-67 (Mki67); green - Mafa (assay MGI:6361002).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 9B No expression in Mafa-expressing cells.
Specimen 9C: embryonic day 15.5 (more )
Note: Double labeled: red - Ki-67 (Mki67); green - Mafb (assay MGI:6361003).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 9C No expression in Mafb-expressing cells.
Specimen 9E: postnatal day 14 (more )
Note: Double labeled: red - Ki-67 (Mki67); green - Nkx6-1 (assay MGI:6361020).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9E
Specimen 9F: postnatal day 14 (more )
Note: Double labeled: red - Ki-67 (Mki67); green - Mafa (assay MGI:6361002).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9F
Specimen 9G: postnatal day 14 (more )
Note: Double labeled: red - Ki-67 (Mki67); green - Mafb (assay MGI:6361003).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9G Expression in higher proportion of Mafb-expressing cells.
Specimen 9H: postnatal day 14 (more )
Note: Triple labeled: red - Ki-67 (Mki67); green - Mafa (assay MGI:6361002); blue - Ins1 (assay MGI:6361014) and Ins2 (assay MGI:6361015).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9H
Specimen 9I: postnatal day 14 (more )
Note: Triple labeled: red - Ki-67 (Mki67); green - Mafb (assay MGI:6361003); blue - Ins1 (assay MGI:6361014) and Ins2 (assay MGI:6361015).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9I Expression in Mafb-expressing cell.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory