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Gene Expression Data
Assay Details
Assay
Reference: J:108721 Nishimura W, et al., A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells. Dev Biol. 2006 May 15;293(2):526-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361016
Gene symbol: Gcg
Gene name: glucagon
Antibody: anti-glucagon
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Texas Red
Results
Specimen 1D: postnatal adult (more )
Note: Double labeled: red - Gcg; green - Mafa (assay MGI:6361002).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1D No co-expression with Mafa.
Specimen 1F: postnatal adult (more )
Note: Double labeled: red - Gcg; green - Mafb (assay MGI:6361003).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1F Co-expression with Mafb.
Specimen 1H: postnatal adult (more )
Note: Double labeled: red - Gcg; green - Maf (cMaf; assay MGI:6361004).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1H Co-expression with cMaf (Maf).
Specimen 4A: embryonic day 10.5 (more )
Note: Triple labeled: red - Gcg; green - Pdx1 (assay MGI:6361018); blue - DAPI.
Structure Level Pattern Image Note
TS17: pancreas primordium Present Regionally restricted 4A Expressing cells easily detected by E10.5
Specimen 4B: embryonic day 10.5 (more )
Note: Triple labeled: red - Gcg; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS17: pancreas primordium Present Regionally restricted 4B Expressing cells easily detected by E10.5
Specimen 4C: embryonic day 10.5 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS17: pancreas primordium Present Regionally restricted 4C
Specimen 4D: embryonic day 11.5 (more )
Note: Triple labeled: red - Gcg; green - Pdx1 (assay MGI:6361018); blue - DAPI.
Structure Level Pattern Image Note
TS19: pancreas primordium Present Regionally restricted 4D
Specimen 4E: embryonic day 11.5 (more )
Note: Triple labeled: red - Gcg; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS19: pancreas primordium Present Regionally restricted 4E
Specimen 4F: embryonic day 11.5 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS19: pancreas primordium Present Regionally restricted 4F
Specimen 4K: embryonic day 15.5 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 4Kb , 4Ka Significant increase in glucagon-expressing cells.
Specimen 4L: postnatal day 5 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 4L
Specimen 4M: postnatal day 8 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 4M
Specimen 4N: postnatal day 14 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 4N
Specimen 5B: embryonic day 15.5 (more )
Note: Triple labeled: red - Gcg; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 5B
Specimen 7D: embryonic day 15.5 (more )
Note: Triple labeled: red - Gcg; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 7D
Specimen 7E: embryonic day 15.5 (more )
Note: Triple labeled: red - Gcg; green - Nkx6-1 (assay MGI:6361020); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 7E Not all glucagon-expression cell express Nkx6-1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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