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Gene Expression Data
Assay Details
Assay
Reference: J:108721 Nishimura W, et al., A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells. Dev Biol. 2006 May 15;293(2):526-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361010
Gene symbol: Ins1
Gene name: insulin I
Antibody: anti-insulin
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Texas Red
Results
Specimen 1C: postnatal adult (more )
Note: Double labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1C Co-expression with Mafa.
Specimen 1E: postnatal adult (more )
Note: Double labeled: red - Ins1 and Ins2; green - Mafb (assay MGI:6361003).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1E No co-expression with Mafb.
Specimen 1G: postnatal adult (more )
Note: Double labeled: red - Ins1 and Ins2; green - Maf (assay MGI:6361004).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1G Co-expression with cMaf (Maf).
Specimen 3B: embryonic day 17.5 (more )
Note: Double labeled: red - Ins1 and Ins2; green - Gcg (assay MGI:6361017).
Structure Level Pattern Image Note
TS25: endocrine pancreas Present Regionally restricted 3B
Specimen 3E: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: endocrine pancreas Present 3E
Specimen E10.5 not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pancreas primordium Absent
Specimen E11.5 not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: pancreas primordium Absent
Specimen 4G: embryonic day 12.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 4G Rare insulin-expressing cells also express Mafa.
Specimen 4H: embryonic day 13.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Pdx1 (assay MGI:6361018); blue - DAPI.
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 4H
Specimen 4I: embryonic day 13.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 4I Rare insulin-expressing cells also express Mafa.
Specimen 4J: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 4Ja , 4Jb Significant increase in insulin-expressing cells.
Specimen 5C: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 5C Several insulin-expressing cells do not express Mafa.
Specimen 6A: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 6A
Specimen 6B: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 6B
Specimen 7A: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 7A
Specimen 7B: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Nkx6-1 (assay MGI:6361020); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 7B All insulin-expressing cells also express Nkx6-1.
Specimen 10A: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 10A
Specimen 10B: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Pax6 (assay MGI:6361023); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 10B
Specimen 11A: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafa (assay MGI:6361002); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 11A
Specimen 11B: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Pdx1 (assay MGI:6361018); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 11B
Specimen 11D: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Mafb (assay MGI:6361003); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 11D
Specimen 11E: embryonic day 15.5 (more )
Note: Triple labeled: red - Ins1 and Ins2; green - Pdx1 (assay MGI:6361018); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 11E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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