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Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296596
Gene symbol: Gcna
Gene name: germ cell nuclear acidic protein
Antibody: TRA98 (ab82527)
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 5A P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - TRA98 (Gcna); green - Sox9 (assay MGI:6296595); blue - DAPI.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 5A P0 CT
Specimen 5A P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - TRA98 (Gcna); green - Sox9 (assay MGI:6296595); blue - DAPI.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 5A P0 KO
Specimen 5A P2 CT: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - TRA98 (Gcna); green - Sox9 (assay MGI:6296595); blue - DAPI.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 5A P2 CT
Specimen 5A P2 KO: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - TRA98 (Gcna); green - Sox9 (assay MGI:6296595); blue - DAPI.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 5A P2 KO
Specimen 5A P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - TRA98 (Gcna); green - Sox9 (assay MGI:6296595); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5A P5 CT
Specimen 5A P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - TRA98 (Gcna); green - Sox9 (assay MGI:6296595); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5A P5 KO
Specimen 6B: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - TRA98 (Gcna); green - Casp3 (assay MGI:6296597); blue - DAPI.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 6B
Specimen 6C: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - TRA98 (Gcna); green - Casp3 (assay MGI:6296597); blue - DAPI.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 6C
Specimen 7B CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: green - TRA98 (Gcna); red - SF1 (Nr5a1 assay MGI:6296584); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present 7B CT
Specimen 7B KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - TRA98 (Gcna); red - SF1 (Nr5a1 assay MGI:6296584); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present 7B KO Authors state expression patterns were indistinguishable between mutant control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory