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Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296597
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved caspase-3
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 6A 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS26: testis Absent 6A 18.5 dpc CT No expressing cells in control testes.
Specimen 6A 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: testis Present 6A 18.5 dpc KO Authors report significant number of expressing cells starting at 18.5 dpc.
Specimen 6A P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS27: testis Absent 6A P0 CT No expressing cells in control testes.
Specimen 6A P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: testis Strong 6A P0 KO Prominent number of expressing cells.
Specimen 6A P2 CT: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS27: testis Absent 6A P2 CT No expressing cells in control testes.
Specimen 6A P2 KO: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: testis Strong 6A P2 KO Prominent number of expressing cells.
Specimen 6A P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Level Pattern Image Note
TS28: testis Absent 6A P5 CT No expressing cells in control testes.
Specimen 6A P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: testis Ambiguous 6A P5 KO
Specimen 6B: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: seminiferous cord Present Regionally restricted 6B Expression in Sertoli cells inside testis cords.
TS27: Sertoli cell Sertoli cell Present Regionally restricted 6B Expression in Sertoli cells inside testis cords.
Specimen 6C: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: germ cell of testis Present Regionally restricted 6C Expression in both germ cells and Sertoli cells inside testis cords.
TS27: seminiferous cord Present Regionally restricted 6C Expression in both germ cells and Sertoli cells inside testis cords.
TS27: Sertoli cell Sertoli cell Present Regionally restricted 6C Expression in both germ cells and Sertoli cells inside testis cords.
Specimen 6D: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - laminin; blue - DAPI.
Structure Level Pattern Image Note
TS27: interstitium of the testis Present 6D Expression in cells in the interstitium.
Specimen 6E: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - laminin; blue - DAPI.
Structure Level Pattern Image Note
TS27: interstitium of the testis Weak 6E Only a few expressing cells in the interstitium.
Specimen 7C CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: green - Casp3; red - Hsd3b (assay MGI:6296592); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Absent 7C CT
Specimen 7C KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Casp3; red - Hsd3b (assay MGI:6296592); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Absent 7C KO Authors state expression patterns were indistinguishable between mutant control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory