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Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296595
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-SOX9
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 3 16.5 dpc CT: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Cell Type Level Pattern Image Note
TS24: seminiferous cord Present 3_16.5 dpc CT
TS24: Sertoli cell Sertoli cell Present 3_16.5 dpc CT
Specimen 3 16.5 dpc KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS24: seminiferous cord Present 3_16.5 dpc KO Authors report Sertoli cell differentiation and testis cord formation were comparable in mutant and control testis.
TS24: Sertoli cell Sertoli cell Present 3_16.5 dpc KO Authors report Sertoli cell differentiation and testis cord formation were comparable in mutant and control testis.
Specimen 3 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Structure Cell Type Level Pattern Image Note
TS26: seminiferous cord Present 3_18.5 dpc CT
TS26: Sertoli cell Sertoli cell Present 3_18.5 dpc CT
Specimen 3 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS26: seminiferous cord Present 3_18.5 dpc KO Authors report Sertoli cell differentiation and testis cord formation were comparable in mutant and control testis.
TS26: Sertoli cell Sertoli cell Present 3_18.5 dpc KO Authors report Sertoli cell differentiation and testis cord formation were comparable in mutant and control testis.
Specimen 5A P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: green - Sox9; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: seminiferous cord Present 5A P0 CT
TS27: Sertoli cell Sertoli cell Present 5A P0 CT
Specimen 5A P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Sox9; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: seminiferous cord Present 5A P0 KO Authors report no significant differences between mutant and control testis.
TS27: Sertoli cell Sertoli cell Present 5A P0 KO Authors report no significant differences between mutant and control testis.
Specimen 5A P2 CT: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: green - Sox9; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: seminiferous cord Present 5A P2 CT
TS27: Sertoli cell Sertoli cell Present 5A P2 CT
Specimen 5A P2 KO: postnatal day 2; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Sox9; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: seminiferous cord Weak 5A P2 KO Decrease in number of proliferating Sertoli cells inside tesis cords.
TS27: Sertoli cell Sertoli cell Weak 5A P2 KO Decrease in number of proliferating Sertoli cells inside tesis cords.
Specimen 5A P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: green - Sox9; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: seminiferous cord Present 5A P5 CT
TS28: Sertoli cell Sertoli cell Present 5A P5 CT
Specimen 5A P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: green - Sox9; red - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS28: seminiferous cord Ambiguous Regionally restricted 5A P5 KO Loss of most of testis cord structures.
TS28: Sertoli cell Sertoli cell Ambiguous Regionally restricted 5A P5 KO Loss of most of testis cord structures.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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