T RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:57262 Song J, et al., The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice. Dev Biol. 1999 Sep 1;213(1):157-69
Assay type:	RNA in situ
MGI Accession ID:	MGI:5491482
Gene symbol:	T
Gene name:	brachyury, T-box transcription factor T

Probe:	T cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Expression was detected using NBT/BCIP for the color reaction.

Results

1G WT ©

Specimen 1G WT: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		1G WT

Specimen 1G IIA-/-IIB-/-: embryonic day 7.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b^tm1Enl (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1G IIA-/-IIB-/-

3A ©

Specimen 3A: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Widespread	3A	Expression along entire primitive streak.

3B ©

Specimen 3B: embryonic day 7.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b⁺ (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	3B	Expression restricted to proximal region.

3C ©

Specimen 3C: embryonic day 7.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b⁺ (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	3C	Expression restricted to proximal region.

3D ©

Specimen 3D: embryonic day 7.5 (more

)
Note: Sagittal section of embryo shown in 3A. Specimen was embedded in plastic.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Widespread	3D	Expression along entire primitive streak.
TS11: node	Present		3D

3E ©

Specimen 3E: embryonic day 7.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b⁺ (more

)
Note: Sagittal section of embryo shown in 3B. Specimen was embedded in plastic.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3E	Expression in primitive streak in proximal epiblast.
TS11: embryo	Present	Regionally restricted	3E	No expression distal region of embryo.

3F ©

Specimen 3F: embryonic day 7.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b⁺ (more

)
Note: Sagittal section of embryo shown in 3C. Specimen was embedded in plastic.

Structure	Level	Image	Note
TS11: primitive streak	Present	3F	Expression in primitive streak in proximal epiblast.
TS11: node	Present	3F
TS11: embryo	Present	3F	No expression distal region of embryo.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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