Probe:	Pyst1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		1A
TS11: embryo mesenchyme	Present		1A	Expression is in the recently formed primary mesenchyme.
TS11: allantois	Present		1A

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1B	Expression is at the mid-/hindbrain boundary.
TS13: future midbrain	Present	Regionally restricted	1B	Expression is at the mid-/hindbrain boundary.
TS13: 2nd branchial arch mesenchyme	Present		1B

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1C	Expression is at the mid-/hindbrain boundary, at the adjacent lateral edge of the neural plate, and in a discrete domain lateral to pro-rhombomere B of the hindbrain.
TS13: future midbrain	Present	Regionally restricted	1C	Expression is at the mid-/hindbrain boundary, at the adjacent lateral edge of the neural plate, and in a discrete domain lateral to pro-rhombomere B of the hindbrain.
TS13: 2nd branchial arch mesenchyme	Present		1C

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		1D
TS13: primitive streak	Present		1D

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		1E
TS13: primitive streak	Present		1E
TS13: neural ectoderm	Weak		1E

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	1F	Expression is at the mid-/hindbrain boundary.
TS14: future midbrain	Present	Regionally restricted	1F	Expression is at the mid-/hindbrain boundary.
TS14: 1st branchial arch	Present		1F
TS14: 1st branchial arch mandibular component	Present		1F
TS14: 2nd branchial arch	Present		1F
TS14: 3rd branchial arch	Present		1F
TS14: forelimb bud mesenchyme	Present		1F
TS14: tail bud	Present		1F
TS14: trunk mesenchyme	Present	Regionally restricted	1F	Expression is in the lateral plate mesoderm at the level of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS14: olfactory placode	Present		1F inset

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	2A	Expression is at the mid-/hindbrain isthmus.
TS15: future midbrain	Present	Regionally restricted	2A	Expression is at the mid-/hindbrain isthmus.
TS15: telencephalon	Present	Regionally restricted	2A	Expression is at the telencephalon-diencephalon boundary and the medial walls of the cerebral hemisphere.
TS15: diencephalon	Present	Regionally restricted	2A	Expression is at the telencephalon-diencephalon boundary.
TS15: head	Present	Regionally restricted	2A	Expression is in the facial process.

Structure	Level	Pattern	Image	Note
TS15: 4th ventricle	Present	Regionally restricted	2B	Expression is in the roof of the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Strong		2C
TS15: hindlimb ridge mesenchyme	Strong		2C
TS15: tail	Present		2C
TS15: trunk somite	Present	Graded	2C	Expression is up-regulated in a craniocaudal sequence.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		2D
TS17: 1st branchial arch	Present		2D
TS17: 2nd branchial arch	Present		2D
TS17: 3rd branchial arch	Present		2D
TS17: 4th branchial arch	Present		2D
TS17: latero-nasal process mesenchyme	Present		2D
TS17: medial-nasal process mesenchyme	Present		2D
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	2D	Expression is at the oral surface.
TS17: forelimb bud mesenchyme	Strong		2D
TS17: hindlimb bud mesenchyme	Strong		2D
TS17: trunk somite	Present	Graded	2D	Expression is up-regulated in a craniocaudal sequence.

Structure	Level	Pattern	Image	Note
TS17: latero-nasal process mesenchyme	Present		2E
TS17: medial-nasal process mesenchyme	Present		2E
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	2E	Expression is at the oral surface.
TS17: Rathke's pouch	Present		2E
TS17: telencephalon	Present	Regionally restricted	2E	Expression is on either side of the midline in the medial walls.

Structure	Level	Pattern	Image	Note
TS17: latero-nasal process mesenchyme	Present		2F
TS17: medial-nasal process mesenchyme	Present		2F
TS17: 1st branchial arch mesenchyme	Present	Regionally restricted	2F	Expression is at the oral surface.
TS17: telencephalon	Present	Regionally restricted	2F	Expression is in the medial walls.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		2G
TS17: tail	Present		2G
TS17: trunk somite	Present	Graded	2G	Expression is up-regulated in a craniocaudal sequence.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2H	Expression is in the distal mesoderm.
TS17: forelimb bud apical ectodermal ridge	Absent		2H

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2I	Expression is in the distal mesenchyme.
TS17: forelimb bud apical ectodermal ridge	Absent		2I
TS17: forelimb bud ectoderm	Absent		2I

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		3A	Expression is at the mid-/hindbrain isthmus.
TS19: midbrain	Present		3A	Expression is at the mid-/hindbrain isthmus.
TS19: latero-nasal process	Present		3A
TS19: medial-nasal process	Present		3A
TS19: telencephalon	Present		3A
TS19: middle ear	Present		3A

Structure	Level	Pattern	Image	Note
TS19: latero-nasal process	Present		3B
TS19: medial-nasal process	Present		3B
TS19: telencephalon	Present	Regionally restricted	3B	Expression is in the medial walls.
TS19: Rathke's pouch	Weak		3B

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		3C
TS19: hindlimb bud	Present		3C
TS19: trunk somite	Present	Regionally restricted	3C	Expression is in the caudal somites.
TS19: tail mesenchyme	Present		3C

Structure	Level	Pattern	Image	Note
TS19: limb	Present	Regionally restricted	3D	Expression is in a narrow rim in the distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: tail dermomyotome	Present	Regionally restricted	3E	There is epaxial and hypaxial myotomal expression.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Present	Regionally restricted	3F	Expression is in the mammary gland primordia.

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present	Regionally restricted	4A	Expression is in the digital tips.
TS20: limb interdigital region	Weak		4A
TS20: tail somite	Weak		4A

Structure	Level	Pattern	Image	Note
TS21: skin	Strong	Regionally restricted	4B	Expression is in hair placodes.
TS21: limb digit	Present	Regionally restricted	4B	Expression is in the tips of the digits.
TS21: tail dermomyotome	Present	Regionally restricted	4B	Expression is in the caudal myotomes.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present	Regionally restricted	4C	Expression is in the tips of the digits.

Structure	Level	Pattern	Image	Note
TS21: lower jaw tooth	Present	Regionally restricted	4D	Expression is in the dental lamina.
TS21: upper jaw tooth	Present	Regionally restricted	4D	Expression is in the dental lamina.
TS21: palatal shelf	Present	Regionally restricted	4D	Expression is in the palatal shelf edges.

Structure	Level	Pattern	Image	Note
TS21: lower jaw incisor	Present	Regionally restricted	4E	Expression is in the dental lamina.
TS21: lower jaw molar	Present	Regionally restricted	4E	Expression is in the dental lamina.
TS21: tongue	Present	Regionally restricted	4E	Expression is in the lateral and medial regions.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	4F	Expression is in the distal whisker placodes.
TS22: naris	Present		4F
TS22: forelimb digit	Present	Regionally restricted	4F	Expression is in the digit tips.

Structure	Level	Pattern	Image	Note
TS22: skin	Strong	Regionally restricted	4G	Expression is in the hair placodes, concentrated in the hypoderm layer of the surface ectoderm (abdominal skin is shown).

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	4H	Expression is in the hair placodes, concentrated in the hypoderm layer of the surface ectoderm (abdominal skin is shown).

Structure	Level	Pattern	Image	Note
TS22: pinna	Present		4I

Structure	Level	Pattern	Image	Note
TS22: upper jaw incisor dental lamina	Present		4J
TS22: upper jaw molar dental lamina	Present		4J
TS22: palatal shelf	Present	Regionally restricted	4J	Expression is around the medial edges of the palatal shelves.
TS22: naris	Present	Regionally restricted	4J	Expression is around the internal nares.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium	Present		4K

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium	Present		4L
TS22: oral region gland	Present	Regionally restricted	4L	Expression is in the parotid salivary gland.