Analysis Tools
cardiovascular system
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• at E9.5, the yolk sac is not properly vascularized
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• by E10.5, about half of homozygous embryos show severe cardiac defects
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craniofacial
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• at E9.5, all homozygous embryos exhibit abnormally shaped heads
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embryo
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• at E9.5, the yolk sac is not properly vascularized
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• by E10.5, about half of homozygous embryos show turning defects
• only a single embryo has turned and appears normal at E10.5
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• at E9.5, all homozygous embryos remain unturned or exhibit incomplete turning
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• at E9.5, all homozygous embryos remain unturned or exhibit incomplete turning
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• by E8.5, homozygous embryos are developmentally delayed, exhibiting some POU5F1/OCT4 staining in the remnant of the primitive streak
• however, all three germ layers are present at E8.5 and germ layer patterning is normal at E9.5
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• by E9.5, homozygous embryos are grossly abnormal
• however, all three germ layers are present at E8.5 and germ layer patterning is normal at E9.5
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• starting at E7.5, homozygous embryos are always smaller than control littermates
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• at E9.5, abnormal head shape is often accompanied by improper cranial neural tube closure
• by E10.5, about half of homozygous embryos show an open cranial neural tube
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• at E9.5, placentas exhibit multiple labyrinth zone defects
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• at E9.5, the Tfeb+ labyrinth trophoblast layer fails to expand
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• at E9.5, Gcm1+ chorioallantoic branching is severely impaired
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• at E9.5, one of 4 homozygous embryos show chorioallantoic attachment; however, less allantoic mesoderm is observed, and the resulting interface fails to expand as widely as in controls
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• at E9.5, three of 4 homozygous embryos show failure of chorioallantoic attachment; allantoic mesoderm is present adjacent to the chorion, but the attachment is weak and easily disrupted during dissection
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growth/size/body
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• at E9.5, all homozygous embryos exhibit abnormally shaped heads
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• by E8.5, homozygous embryos are developmentally delayed, exhibiting some POU5F1/OCT4 staining in the remnant of the primitive streak
• however, all three germ layers are present at E8.5 and germ layer patterning is normal at E9.5
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• starting at E7.5, homozygous embryos are always smaller than control littermates
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mortality/aging
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IMPC - JAX
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• homozygous embryos are present in Mendelian ratios at E7.5-E9.5 but absent at E12.5; by E10.5, more than half of homozygous embryos are dying/undergoing resorption
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nervous system
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• at E9.5, abnormal head shape is often accompanied by improper cranial neural tube closure
• by E10.5, about half of homozygous embryos show an open cranial neural tube
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