Analysis Tools
cardiovascular system
|
• at E10.5, most homozygous embryos that are alive exhibit heart defects
|
hemorrhage
(
J:374598
)
|
• at E10.5, some homozygous embryos exhibit abnormal blood pooling
|
craniofacial
|
• at E10.5, half of the homozygous embryos exhibit an abnormal head shape
|
embryo
|
• by E11.5, most homozygous embryos are abnormal
• however, germ layer development is normal at E9.5 and, surprisingly, a few embryos are still normal in appearance at E11.5
|
|
• at E9.5, homozygous embryos are generally smaller but indistinguishable from control littermates
• at E10.5 and E11.5, all recovered homozygous embryos are smaller than controls
|
|
• at E10.5, placentas exhibit moderate labyrinth zone defects with incomplete penetrance; severity of labyrinth zone defects correlates with that of the embryonic phenotype
• one of 4 placentas show normal labyrinth zone development at E10.5
|
|
• at E10.5, two of 4 placentas exhibit a markedly reduced Tfeb+ labyrinth trophoblast layer
|
|
• at E10.5, all (4 of 4) placentas show a consistently reduced Tpbpa+ spongiotrophoblast layer
|
|
• at E10.5, three of 4 placentas show impaired chorioallantoic branching, as indicated by reduced Gcm1 expression
• however, placentas are properly developed at E9.5
|
growth/size/body
|
• at E10.5, half of the homozygous embryos exhibit an abnormal head shape
|
|
• at E9.5, homozygous embryos are generally smaller but indistinguishable from control littermates
• at E10.5 and E11.5, all recovered homozygous embryos are smaller than controls
|
mortality/aging
|
IMPC - JAX
|
|
IMPC - JAX
|
|
• approximately 1/3 of homozygous embryos recovered at E10.5 are dying or resorbed while those that are still alive display a range of defects; two homozygous embryos recovered at E12.5 are dying/undergoing resorption
|
