Phenotypes associated with this allele

• Allele Symbol: Pold3^em1Linl
• Allele Name: endonuclease-mediated mutation 1, Lin Liu
• Allele ID: MGI:6195840
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pold3^em1Linl/Pold3^em1Linl	involves: C57BL/6 * DBA/2	MGI:6258927
ht2	Pold3^em1Linl/Pold3^+	involves: C57BL/6 * DBA/2	MGI:6258928

Genotype
MGI:6258927

hm1

• Allelic Composition: Pold3^em1Linl/Pold3^em1Linl
• Genetic Background: involves: C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:263620 )

• mice die between E4.5 and E7.5

embryo

abnormal trophoblast giant cell proliferation ( J:263620 )

• reduced proliferation during blastocyst outgrowth

absent egg cylinders ( J:263620 )

abnormal embryonic tissue physiology ( J:263620 )

• in culture, inner cell mass fail to expand unlike wild-type cells

• increased apoptosis during blastocyst outgrowth

decreased inner cell mass proliferation ( J:263620 )

cellular

decreased inner cell mass proliferation ( J:263620 )

abnormal trophoblast giant cell proliferation ( J:263620 )

• reduced proliferation during blastocyst outgrowth

Genotype
MGI:6258928

ht2

• Allelic Composition: Pold3^em1Linl/Pold3⁺
• Genetic Background: involves: C57BL/6 * DBA/2

reproductive system

decreased male germ cell number ( J:263620 )

♂ • with age

arrest of male meiosis ( J:263620 )

♂ • middle-aged mice exhibit impaired male meiosis progression with shortened telomeres and increased percentage of spermatocyte in leptotene and zygotene stages compared with wild-type mice

increased male germ cell apoptosis ( J:263620 )

♂ • with age

seminiferous tubule degeneration ( J:263620 )

♂ • more pronounced with age

small testis ( J:263620 )

♂ • in old, but not middle-aged, mice

decreased testis weight ( J:263620 )

♂ • in old, but not middle-aged, mice

homeostasis/metabolism

impaired double-strand DNA break repair ( J:263620 )

• impaired meiotic DNA repair and recombination in spermatocyte of middle-aged mice

cellular

decreased male germ cell number ( J:263620 )

♂ • with age

decreased telomere length ( J:263620 )

♂ • shortened telomeres in the male germ cells of middle-aged mice

arrest of male meiosis ( J:263620 )

♂ • middle-aged mice exhibit impaired male meiosis progression with shortened telomeres and increased percentage of spermatocyte in leptotene and zygotene stages compared with wild-type mice

increased male germ cell apoptosis ( J:263620 )

♂ • with age

impaired double-strand DNA break repair ( J:263620 )

• impaired meiotic DNA repair and recombination in spermatocyte of middle-aged mice

spontaneous chromosome breakage ( J:263620 )

♂ • in spermatocyte of middle-aged mice

endocrine/exocrine glands

seminiferous tubule degeneration ( J:263620 )

♂ • more pronounced with age

small testis ( J:263620 )

♂ • in old, but not middle-aged, mice

decreased testis weight ( J:263620 )

♂ • in old, but not middle-aged, mice