Analysis Tools
mortality/aging
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• heterozygous crosses usually result in ~50% of the expected Mendelian ratio at P10
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respiratory system
chylothorax
(
J:221521
)
cardiovascular system
| N |
• surviving 8 week old mice exhibit normal systolic arterial pressure and pulse rate
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• adult aortic ring endothelial cells fail to form microtubes in Matrigel after 3 weeks in culture, unlike in wild-type explants cultures
• aortic ring explants exhibit degenerated endothelial tip cells with retraction bulbs and lack filopodia, unlike wild-type explants where interconnections between endothelial tubes and filopodia appear intact
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• mice show a mild and transient reduction in retinal primary plexus vascularization at P4, P6, and P8; however, delay in radial outgrowth of the primary plexus is overcome at P10, as shown by absence of avascular zones in the primary retinal periphery and normally formed deep plexi
• delayed angiogenic fronts show no changes in tip cell morphology or number and length of tip cell filopodia relative to wild-type controls
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• as early as 4 weeks of age, all mice exhibit abnormally shaped tortuous retinal arteries, unlike wild-type controls
• incidence (%) of tortuous arteries increases with age, with ~62% observed at 1 month and ~85% at 4 months of age or later
• adult tortuous arteries are significantly (6%) longer than wild-type arteries
• however, no changes in adult retinal artery width or neovascularization are observed, and both retinal artery and vein width is normal at P6 and P8
• proliferative activity of retinal arteries and veins is normal at P6, as measured by Ki67 staining
• retinal blood vessels show normal smooth muscle coverage and no significant vessel regression is observed at P6
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• mice exhibit a transient decrease in retinal angiogenesis during early postnatal stages that is overcome by P10
• in vitro microvessel sprouting and microtube formation from P10 and adult aortic ring explants are severely reduced
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vision/eye
| N |
• adult mice show normal retinal layering and layer thickness as well as normal retinal function relative to wild-type controls
• no defects in astrocyte migration towards the retinal periphery are noted at P4, P6 and P8
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• mice show a mild and transient reduction in retinal primary plexus vascularization at P4, P6, and P8; however, delay in radial outgrowth of the primary plexus is overcome at P10, as shown by absence of avascular zones in the primary retinal periphery and normally formed deep plexi
• delayed angiogenic fronts show no changes in tip cell morphology or number and length of tip cell filopodia relative to wild-type controls
|
|
• as early as 4 weeks of age, all mice exhibit abnormally shaped tortuous retinal arteries, unlike wild-type controls
• incidence (%) of tortuous arteries increases with age, with ~62% observed at 1 month and ~85% at 4 months of age or later
• adult tortuous arteries are significantly (6%) longer than wild-type arteries
• however, no changes in adult retinal artery width or neovascularization are observed, and both retinal artery and vein width is normal at P6 and P8
• proliferative activity of retinal arteries and veins is normal at P6, as measured by Ki67 staining
• retinal blood vessels show normal smooth muscle coverage and no significant vessel regression is observed at P6
|
homeostasis/metabolism
chylothorax
(
J:221521
)
