Phenotypes associated with this allele

• Allele Symbol: Sqstm1^tm2.1Jmos
• Allele Name: targeted mutation 2.1, Jorge Moscat
• Allele ID: MGI:5487454
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd	MGI:5487464
cn2	Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos Tg(Nes-cre)1Kln/0	involves: C57BL/6 * DBA	MGI:5487466
cn3	Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: C57BL/6 * DBA	MGI:5487467
cn4	Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos Tg(Fabp4-cre)1Rev/0	Not Specified	MGI:5487465

Genotype
MGI:5487464

cn1

• Allelic Composition: Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

growth/size/body region phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal body weight and composition

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal glucose tolerance

cellular

cellular phenotype ( J:194291 )

N • brown adipose tissue exhibits normal mitochondrial function (measured by cox activity)

Genotype
MGI:5487466

cn2

• Allelic Composition: Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: C57BL/6 * DBA

growth/size/body

growth/size/body region phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal body weight and composition

behavior/neurological

behavior/neurological phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal food intake

Genotype
MGI:5487467

cn3

• Allelic Composition: Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: C57BL/6 * DBA

growth/size/body

growth/size/body region phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal body weight and composition

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal glucose tolerance

liver/biliary system

liver/biliary system phenotype ( J:194291 )

N • mice do not exhibit liver steatosis

behavior/neurological

behavior/neurological phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal food intake

Genotype
MGI:5487465

cn4

• Allelic Composition: Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos; Tg(Fabp4-cre)1Rev/0
• Genetic Background: Not Specified

Enhanced lipid deposition with increased lipid droplet sizes of brown adipose tissue in Sqstm1^tm2.1Jmos/Sqstm1^tm2.1Jmos Tg(Fabp4-cre)1Rev/0 mice

homeostasis/metabolism

decreased core body temperature ( J:194291 )

• at room temperature and during acute cold exposure

decreased energy expenditure ( J:194291 )

impaired glucose tolerance ( J:194291 )

• in mice fed standard chow but not a high fat diet

insulin resistance ( J:194291 )

• in mice fed standard chow or a high fat diet

adipose tissue

increased total body fat amount ( J:194291 )

• in mice fed standard chow

• however, fat mass is normal in mice fed a high fat diet

increased brown fat cell lipid droplet size ( J:194291 )

increased white fat cell size ( J:194291 )

abnormal brown adipose tissue thermogenesis ( J:194291 )

• impaired nonshivering thermogenesis above brown adipose tissue treated with the selective beta-3 adrenergic receptor agonist CL-316,243

cellular

abnormal mitochondrial crista morphology ( J:194291 )

• fewer and shorter cristae displaced to the periphery with varying degrees of disorientation in the mitochondria of brown adipose tissue

disorganized mitochondrial cristae ( J:194291 )

• in the mitochondria of brown adipose tissue

abnormal mitochondrial ATP synthesis coupled electron transport ( J:194291 )

• decreased oxidative capacity in the mitochondria of brown adipose tissue

growth/size/body

increased lean body mass ( J:194291 )

• in mice fed a high fat diet

• however, lean mass is normal in mice fed standard chow

increased body weight ( J:194291 )

• in mice fed standard chow or a high fat diet

liver/biliary system

hepatic steatosis ( J:194291 )

• in mice fed standard chow or a high fat diet

behavior/neurological

behavior/neurological phenotype ( J:194291 )

N • mice fed standard chow or a high fat diet exhibit normal food intake

decreased locomotor activity ( J:194291 )

• reduced locomotor activity (total and mean dark but not mean light)