Analysis Tools|
Allele Symbol Allele Name Allele ID |
Med23tm1Gwan targeted mutation 1, Gang Wang MGI:5441008 |
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| Summary |
5 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• adipose derived stem cells (ADSCs) infected with a cre expressing adenovirus and exposed to an adipogenic hormone cocktail accumulate fewer lipids and show attenuated expression of adipocyte markers and enhanced expression of smooth muscle cell markers
• differentiation of ADSCs, infected with a cre expressing adenovirus and treated with TGFB, into smooth muscle cells is enhanced
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are present in approximate Mendelian ratios at P1 (21% versus expected 25%) and show no obvious phenotypic abnormalities
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice die between E10.5 and E13.5; only 2 out of 14 embryos (14.29%) are recovered at E13.5 and are both resorbed
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• E13.5 embryos exhibit necrosis
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• intracranial vasculature is poorly developed at E9.5 and intracranial capillaries are dilated in the forehead at E10.5
• at E9.5, H&E staining shows a significant increase in blood vessel lumen size in the head region
• at E9.5, whole-mount IF staining with an antibody against PECAM (an endothelial cell marker) shows vascular dilation and remodeling defects of the dendritic capillary plexus in the middle head region
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• at E12.5, embryos exhibit upregulation of Angpt2 (angiopoietin 2, an inhibitor of vascular stability) in the head region, contributing to cranial vascular defects, edema and hemorrhage
• however, the intersomitic vasculature, dorsal aorta, and endocardium are all well developed at E12.5
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• at E9.5, PECAM staining indicates remodeling defects of the dendritic capillary plexus in the middle head region
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• at E9.5, PECAM staining indicates a diminished vascular network in the head region
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• at E9.5, H&E staining shows a significant increase in blood vessel lumen size in the head region
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• intracranial capillaries are dilated in the forehead at E10.5
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• ~70% of embryos exhibit intracranial hemorrhage starting from E10.5
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• ~70% of embryos exhibit intracranial hemorrhage starting from E10.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in vivo retinal angiogenesis assays show that mice injected with tamoxifen from P1 to P3 exhibit significantly reduced numbers of tip cells and filopodia at the edge of the vascular network in retinal tissues at P5
• at P5, PECAM staining shows a reduction in vascular network density as well as a decreased staining signal suggesting reduced levels of PECAM protein in retinal vessels
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• surprisingly, mice survive normally and exhibit normal heart morphology with no obvious histological defects at P30
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/07/2026 MGI 6.24 |
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