Phenotypes associated with this allele

• Allele Symbol: Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf
• Allele Name: transgene insertion, Veronique Lefebvre
• Allele ID: MGI:5426519
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Sox9^tm1Gsr/Sox9^tm1Gsr Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf/0	involves: 129P2/OlaHsd * C57BL/6	MGI:5426540
cn2	Adgrg6^tm1Arte/Adgrg6^tm1Arte Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf/0	involves: 129S5/SvEvBrd	MGI:7266839
cn3	Prmt5^tm2c(EUCOMM)Wtsi/Prmt5^tm2c(EUCOMM)Wtsi Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf/0	involves: C57BL/6N	MGI:7277816

Genotype
MGI:5426540

cn1

• Allelic Composition: Sox9^tm1Gsr/Sox9^tm1Gsr; Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal epiphyseal plate morphology ( J:183084 )

• with doxycycline treatment from E15.5 on, columnar zones are shortened in growth plates of mutants

chondrodystrophy ( J:183084 )

• with doxycycline treatment from E15.5 onward, severe dwarfism results

abnormal endochondral bone ossification ( J:183084 )

• endochondral bones stop elongating by E17.5 with doxycycline treatment from E15.5 onward

abnormal chondrocyte physiology ( J:183084 )

• chondrocytes lose ability to enlarge by E17.5 when doxycycline treatment takes place from E15.5 onward

• columnar cells stop proliferating at a rate 3 times greater than control cells with doxycycline treatment starting at E15.5; number of apoptotic cells in the columnar zone of growth plates is about 28 fold higher and about 5 times higher in the prehypertrophic zone of controls with 2 days of doxycycline treatment

Genotype
MGI:7266839

cn2

• Allelic Composition: Adgrg6^tm1Arte/Adgrg6^tm1Arte; Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf/0
• Genetic Background: involves: 129S5/SvEvBrd

skeleton

abnormal intervertebral disk morphology ( J:313834 )

• altered extracellular matrix component expression and endplate-oriented disk herniations at 8 months of age

scoliosis ( J:313834 )

• embryonic induction of recombinase induction resulted in scoliosis in 25% and 16.7% of mice with Cobb angles of 11 to 43 degrees at P20 and P180, respectively

Genotype
MGI:7277816

cn3

• Allelic Composition: Prmt5^{tm2c(EUCOMM)Wtsi}/Prmt5^{tm2c(EUCOMM)Wtsi}; Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf/0
• Genetic Background: involves: C57BL/6N

skeleton

skeleton phenotype ( J:283043 )

N • no signs of scoliosis are seen following induction of recombinase expression at either 2 or 4 weeks of age

abnormal intervertebral disk morphology ( J:283043 )

• at 4 months of age, 3 months post induction of recombinase expression, there is a minor increase in acellular clefts at the midline of the endplate

• however, no gross signs of degenerative histopathology are seen