Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf
Transgene Detail

Summary

• Symbol: Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf
• Name: transgene insertion, Veronique Lefebvre
• MGI ID: MGI:5426519
• Synonyms: ATC
• Transgene: Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf Location: unknown
• Alliance: Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Acan

• Mutation details: A bigenic construct was generated with a 0.9kb fragment of the Col2a1 promoter, 4 tandem copies of a cartilage-specific Acan enhancer in an intron, a splice acceptor site, the reverse tetracycline transactivator coding sequence and a polyadenylation site composing the first gene. A 4 kb Trp53 intron insulator sequence separated the rtTA sequence from the second part of the construct, consisting of a heptad of tandem copies of a tetracycline-responsive element (tetO), the human cytomegalovirus promoter (hCMV), the cre recombinase coding sequence, and a polyadenylation site. No founder information for the transgene is available. Upon treatment of transgenic mice with doxycycline, the rtTA is activated and binds to tetO to induce cre expression. (J:183084)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Acan (species not specified) Summary of all recombinase alleles driven by Acan.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:183084 Dy P, et al., Sox9 directs hypertrophic maturation and blocks osteoblast differentiation of growth plate chondrocytes. Dev Cell. 2012 Mar 13;22(3):597-609
• All: 12 reference(s)