Phenotypes associated with this allele

• Allele Symbol: Gmnn^tm1Tjm
• Allele Name: targeted mutation 1, Thomas J MacGarry
• Allele ID: MGI:4999617
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gmnn^tm1Tjm/Gmnn^tm1Tjm	involves: 129/Sv * C57BL/6 * C57BL/6J	MGI:4999651
cn2	Gmnn^tm1Tjm/Gmnn^tm1Tjm Tg(Mx1-cre)1Cgn/0	involves: 129/Sv * C57BL/6 * C57BL/6J * CBA	MGI:4999652
cn3	Gmnn^tm1Tjm/Gmnn^tm1Tjm Tg(Nes-cre)1Kln/0	involves: 129/Sv * C57BL/6 * SJL	MGI:4999656
cn4	Gmnn^tm1Tjm/Gmnn^+ Tg(Nes-cre)1Kln/0	involves: 129/Sv * C57BL/6 * SJL	MGI:4999657

Genotype
MGI:4999651

hm1

• Allelic Composition: Gmnn^tm1Tjm/Gmnn^tm1Tjm
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:171741 )

• mice are viable and fertile

Genotype
MGI:4999652

cn2

• Allelic Composition: Gmnn^tm1Tjm/Gmnn^tm1Tjm; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J * CBA

mortality/aging

premature death ( J:171741 )

• most mice die by 3 weeks after pIpC treatment

hematopoietic system

decreased hematopoietic stem cell proliferation ( J:171741 )

• hematopoietic stem cells and progenitor cells from pIpC-treated mice exhibit reduced proliferation in vitro compared with control cells

• however, cells from pIpC-treated mice exhibit normal hematopoietic stem cells maintenance

anemia ( J:171741 )

• profound and progressive in pIpC-treated mice

abnormal bone marrow cell morphology/development ( J:171741 )

• hematopoietic stem cells and progenitor cells from pIpC-treated mice fail to form erythroid colonies on methylcellulose unlike control cells

• hematopoietic stem cells and progenitor cells from pIpC-treated mice for fewer, smaller granulocyte and monocyte colonies on methylcellulose compared with control cells

• however, cells from pIpC-treated mice exhibit normal myeloid colony formation and hematopoietic stem cells maintenance

abnormal common myeloid progenitor cell morphology ( J:171741 )

• cells from pIpC-treated mice for fewer granulocyte and monocyte colonies on methylcellulose compared with control cells

• however, cells from pIpC-treated mice exhibit normal myeloid colony formation

decreased bone marrow cell number ( J:171741 )

• one third of control after pIpC treatment

increased megakaryocyte cell number ( J:171741 )

• pIpC-treated mice exhibit a 10-fold increased CD41+ megakaryocytes in the marrow compared with control mice

abnormal common lymphocyte progenitor cell morphology ( J:171741 )

• white blood cell precursor cells from pIpC-treated mice exhibit over-replication of DNA compared with control cells

decreased erythroid progenitor cell number ( J:171741 )

• in the splenic red pulp of mice treated with pIpC

absent erythroid progenitor cell ( J:171741 )

• hematopoietic stem cells and progenitor cells from pIpC-treated mice fail to form erythroid colonies on methylcellulose unlike control cells

abnormal megakaryocyte progenitor cell morphology ( J:171741 )

• pIpC-treated mice exhibit an increase in Lin- Sca1- c-Kit+ CD9+ CD41+ megakaryocyte precursors compared with control mice

• marrow from pIpC-treated mice forms 3 times as many megakaryocyte colonies on methylcellulose due to increased differentiation rather than increased number of progenitor cells compared with control cells

abnormal proerythroblast morphology ( J:171741 )

• pIpC-treated mice exhibit reduced proerythroblasts, basophilic erythroplasts, polychromatophiolic erythroblasts, orthochromatophilic erythroblasts, and reticulocytes compared with control mice

decreased erythrocyte cell number ( J:171741 )

• pIpC-treated mice exhibit 40%-60% less red blood cells than control mice

thrombocytosis ( J:171741 )

• 5 to 10 times in pIpC-treated mice

decreased leukocyte cell number ( J:171741 )

• in pIpC-treated mice

decreased granulocyte number ( J:171741 )

• pIpC-treated mice exhibit a decrease in Gr1+ Mac1+ leukocytes in the marrow compared with control mice

decreased neutrophil cell number ( J:171741 )

• in pIpC-treated mice

reticulocytopenia ( J:171741 )

increased hematopoietic stem cell number ( J:171741 )

• pIpC-treated mice exhibit an increase in Lin- Sca1+ c-Kit+ stem cells compared with control mice

abnormal spleen red pulp morphology ( J:171741 )

• paucicellular

abnormal bone marrow cell physiology ( J:171741 )

• bone marrow cells from pIpC-treated mice exhibit poor engraftment compared with control cells

homeostasis/metabolism

increased circulating erythropoietin level ( J:171741 )

• pIpC-treated mice exhibit increased serum erythropoietin levels compared with control mice

• however, serum thrombopoietin levels are normal

immune system

decreased leukocyte cell number ( J:171741 )

• in pIpC-treated mice

decreased granulocyte number ( J:171741 )

• pIpC-treated mice exhibit a decrease in Gr1+ Mac1+ leukocytes in the marrow compared with control mice

decreased neutrophil cell number ( J:171741 )

• in pIpC-treated mice

abnormal spleen red pulp morphology ( J:171741 )

• paucicellular

cellular

decreased hematopoietic stem cell proliferation ( J:171741 )

• hematopoietic stem cells and progenitor cells from pIpC-treated mice exhibit reduced proliferation in vitro compared with control cells

• however, cells from pIpC-treated mice exhibit normal hematopoietic stem cells maintenance

Genotype
MGI:4999656

cn3

• Allelic Composition: Gmnn^tm1Tjm/Gmnn^tm1Tjm; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129/Sv * C57BL/6 * SJL

mortality/aging

mortality/aging ( J:171716 )

N • mice exhibit normal survival

reproductive system

decreased litter size ( J:171716 )

• slightly

nervous system

nervous system phenotype ( J:171716 )

N • mice exhibit normal neuroanatomy

N • neuron stem cells exhibit normal proliferation and differentiation

N • neurosphere cells exhibit normal DNA replication

behavior/neurological

behavior/neurological phenotype ( J:171716 )

N • mice exhibit normal locomotion, feeding, avoidance behavior, and response to noxious stimuli

Genotype
MGI:4999657

cn4

• Allelic Composition: Gmnn^tm1Tjm/Gmnn⁺; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129/Sv * C57BL/6 * SJL

reproductive system

decreased litter size ( J:171716 )

• slightly from male mice

• however, female mice produce normal litters