All Phenotypes Vrk1<Gt(RRR178)Byg> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	B6.129P2-Vrk1^{Gt(RRR178)Byg}	MGI:5910363
hm2	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	FVB.129P2(B6)-Vrk1^{Gt(RRR178)Byg}	MGI:6865660
hm3	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	involves: 129P2/OlaHsd * C57BL/6	MGI:4421834
cx4	Trp53^tm1Tyj/Trp53^tm1Tyj Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6	MGI:6865653

Allelic Composition	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}
Genetic Background	B6.129P2-Vrk1^{Gt(RRR178)Byg}

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vrk1^{Gt(RRR178)Byg} mutation (0 available); any Vrk1 mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

early reproductive senescence ( J:171525 )

• each of the two males tested produced one litter at 42 days postpartum (dpp); however, both males failed to produce a second litter by 98 dpp

reproductive system

abnormal oocyte morphology ( J:207272 )

• 3-D transparent images of the antral follicle and the Graffian follicle showed that the sizes of oocytes are smaller than those in wild-type follicles

• at 6 weeks of age, oocyte volumes in antral and Graffian follicles are decreased by 42% and 37%, respectively, indicating defects in oocyte quality at the preovulatory stage

decreased secondary ovarian follicle number ( J:207272 )

• average number of pre-antral follicles (equal to or larger than 50um in diameter) per ovary is reduced by 36%, 43% and 38%, respectively, in 6-, 16- and 43-week-old females

decreased tertiary ovarian follicle number ( J:207272 )

• average number of antral follicles per ovary is reduced by 47%, 22% and 46%, respectively, in 6-, 16- and 43-week-old females

• however, the volume of the antrum cavity in Graffian follicles is not altered at any age

impaired ovarian folliculogenesis ( J:207272 )

• expression levels of 17 genes involved in both early and later folliculogenesis are significantly decreased, suggesting defects in folliculogenesis

abnormal ovary tissue architecture ( J:207272 )

• at 6 weeks of age, 3-D segmented images of the whole ovary showed that the volume fraction of the stroma is increased from 56% to 64% relative to the wild-type ovary

early reproductive senescence ( J:171525 )

• each of the two males tested produced one litter at 42 days postpartum (dpp); however, both males failed to produce a second litter by 98 dpp

female infertility ( J:171525 )

• six-week-old females fail to produce litters despite the presence of copulatory plugs

male infertility ( J:171525 )

• males fail to produce a second litter after 42 dpp

cellular

abnormal oocyte morphology ( J:207272 )

• 3-D transparent images of the antral follicle and the Graffian follicle showed that the sizes of oocytes are smaller than those in wild-type follicles

• at 6 weeks of age, oocyte volumes in antral and Graffian follicles are decreased by 42% and 37%, respectively, indicating defects in oocyte quality at the preovulatory stage

endocrine/exocrine glands

decreased secondary ovarian follicle number ( J:207272 )

• average number of pre-antral follicles (equal to or larger than 50um in diameter) per ovary is reduced by 36%, 43% and 38%, respectively, in 6-, 16- and 43-week-old females

decreased tertiary ovarian follicle number ( J:207272 )

• average number of antral follicles per ovary is reduced by 47%, 22% and 46%, respectively, in 6-, 16- and 43-week-old females

• however, the volume of the antrum cavity in Graffian follicles is not altered at any age

impaired ovarian folliculogenesis ( J:207272 )

• expression levels of 17 genes involved in both early and later folliculogenesis are significantly decreased, suggesting defects in folliculogenesis

abnormal ovary tissue architecture ( J:207272 )

• at 6 weeks of age, 3-D segmented images of the whole ovary showed that the volume fraction of the stroma is increased from 56% to 64% relative to the wild-type ovary

Allelic Composition	Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}
Genetic Background	FVB.129P2(B6)-Vrk1^{Gt(RRR178)Byg}

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Vrk1^{Gt(RRR178)Byg} mutation (0 available); any Vrk1 mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

female infertility ( J:171525 )

• six-week-old females fail to produce litters despite the presence of copulatory plugs

male infertility ( J:171525 )

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased male germ cell number ( J:156653 , J:171525 )

• progressive loss of meitotic cells and spermatids with age (J:156653)

• however, the earliest spermatogonia are retained (J:156653)

• significant loss of male germ cells at all stages of meiosis and differentiation by 14 weeks of age (J:171525)

abnormal oogenesis ( J:171525 )

• females exhibit multiple defects during oocyte development, including delays in meiotic progression and lagging chromosomes during metaphase II

• however, cultured germinal vesicle (GV) oocytes obtained from antral follicles show no gross defects in histone H2A Thr120 and histone H3 Ser10 phosphorylation

abnormal female meiosis ( J:171525 )

• in vitro, many oocytes obtained from the ampulla of superovulated females fail to reach MII arrest

• only 15.6% of GV stage oocytes reach the "parachute" stage versus 49.1% in wild-type females; however, normal numbers of oocytes are found at later stages (prometaphase and metaphase I)

• only 22.5% of GV stage oocytes ultimately reach metaphase II versus 54.5% in wild-type females

• 42.9% of those GV stage oocytes that reach metaphase II exhibit lagging chromosomes versus 2.7% in wild-type females; as a result, less than 13% of oocytes reach metaphase II properly

• however, spindle formation and spindle length remain normal and the overall chromosome number is unaffected

impaired polar body extrusion ( J:171525 )

• in vitro, only 27.9% of GV stage oocytes obtained from antral follicles of superovulated females show polar body extrusion versus 59.9% in wild-type females; 67.7% progress through germinal vesicle breakdown (GVBD) but fail to extrude a polar body versus only 39.2% in wild-type females, indicating meiotic delays

abnormal seminiferous tubule morphology ( J:156653 )

• as early as 6 weeks of age, a striking loss of cellular density is seen in many tubules with only Sertoli cells present

• by 11 weeks of age all tubules contain only Sertoli cells

seminiferous tubule degeneration ( J:156653 , J:171525 )

(J:156653)

• marked loss/degeneration of the seminiferous tubular epithelium by 14 weeks of age (J:171525)

small testis ( J:156653 )

• decrease in the size of the testis in post pubescent mice, although the overall body sizes of all mice are comparable

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age

early reproductive senescence ( J:171525 )

• all males are fertile at the onset of sexual maturity but become sterile by 10 weeks of age

abnormal spermatogenesis ( J:156653 , J:171525 )

• largely devoid of GCNA+ cells in tubules, a marker of spermatogonial stem cells, mitotic spermatogonia, and cells in the prediplotene stages of meiosis I (J:156653)

• PCNA + cells, a marker of proliferating type A and B spermatogonia, are present at 4 weeks but by 14 weeks, 90% of tubules devoid (J:156653)

• adult mice are devoid of KIT+, an early spermatogonial differentiation marker, cells (J:156653)

• progressive reduction of all stages of spermatogenesis within testes, starting at 10 weeks of age (J:171525)

• no meiotic arrest or defects in synaptonemal complex formation are observed (J:171525)

azoospermia ( J:156653 )

abnormal spermatid morphology ( J:156653 )

• progressive loss of spermatids with age

abnormal spermatogonia proliferation ( J:156653 )

• progressive loss of proliferating spermatogonia

abnormal epididymis morphology ( J:156653 )

• at 6 weeks of age only 20% of lumens have sperm

• at 12 weeks of age, the lumens of the epididymal tubules are devoid of sperm

female infertility ( J:156653 , J:171525 )

(J:156653)

• females fail to produce litters or show any signs of pregnancy (J:171525)

• however, deposition of seminal fluid is always observed, and ovaries contain all stages of folliculogenesis (including corpora lutea) indicating successful ovulation (J:171525)

male infertility ( J:156653 , J:171525 )

• in fertility tests, only 1 of 9 males produce a single litter, all of the other males fail to produce any litters (J:156653)

• all males are sterile by 10 weeks of age; males never sire a litter after 69 days postpartum (dpp), despite continued presence of copulatory plugs (J:171525)

impaired fertilization ( J:171525 )

• in vitro, most oocytes obtained from the ampulla of superovulated females show absence of maternal and paternal pronuclei and no embryonic development, indicating failure of fertilization

• some oocytes progress to the 2-cell stage but not beyond, and all oocytes degenerate after 96 hours in culture

cellular

abnormal spermatid morphology ( J:156653 )

• progressive loss of spermatids with age

decreased male germ cell number ( J:156653 , J:171525 )

• progressive loss of meitotic cells and spermatids with age (J:156653)

• however, the earliest spermatogonia are retained (J:156653)

• significant loss of male germ cells at all stages of meiosis and differentiation by 14 weeks of age (J:171525)

azoospermia ( J:156653 )

abnormal oogenesis ( J:171525 )

• females exhibit multiple defects during oocyte development, including delays in meiotic progression and lagging chromosomes during metaphase II

• however, cultured germinal vesicle (GV) oocytes obtained from antral follicles show no gross defects in histone H2A Thr120 and histone H3 Ser10 phosphorylation

abnormal female meiosis ( J:171525 )

• in vitro, many oocytes obtained from the ampulla of superovulated females fail to reach MII arrest

• only 15.6% of GV stage oocytes reach the "parachute" stage versus 49.1% in wild-type females; however, normal numbers of oocytes are found at later stages (prometaphase and metaphase I)

• only 22.5% of GV stage oocytes ultimately reach metaphase II versus 54.5% in wild-type females

• 42.9% of those GV stage oocytes that reach metaphase II exhibit lagging chromosomes versus 2.7% in wild-type females; as a result, less than 13% of oocytes reach metaphase II properly

• however, spindle formation and spindle length remain normal and the overall chromosome number is unaffected

impaired polar body extrusion ( J:171525 )

abnormal spermatogonia proliferation ( J:156653 )

• progressive loss of proliferating spermatogonia

mortality/aging

early reproductive senescence ( J:171525 )

• all males are fertile at the onset of sexual maturity but become sterile by 10 weeks of age

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:156653 )

• as early as 6 weeks of age, a striking loss of cellular density is seen in many tubules with only Sertoli cells present

• by 11 weeks of age all tubules contain only Sertoli cells

seminiferous tubule degeneration ( J:156653 , J:171525 )

(J:156653)

• marked loss/degeneration of the seminiferous tubular epithelium by 14 weeks of age (J:171525)

small testis ( J:156653 )

• decrease in the size of the testis in post pubescent mice, although the overall body sizes of all mice are comparable

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age

Allelic Composition	Trp53^tm1Tyj/Trp53^tm1Tyj Vrk1^{Gt(RRR178)Byg}/Vrk1^{Gt(RRR178)Byg}
Genetic Background	involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trp53^tm1Tyj mutation (12 available); any Trp53 mutation (232 available)
Vrk1^{Gt(RRR178)Byg} mutation (0 available); any Vrk1 mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

decreased tumor-free survival time ( J:171525 )

• mice begin to succumb to tumors around 3 months of age, as seen in single Trp53^tm1Tyj homozygotes

early reproductive senescence ( J:171525 )

• all three males tested produced one litter between 35 and 50 days postpartum (dpp), and two sired litters after 50 dpp (one at 68 dpp and one at 69 dpp); however, males become sterile after 69 dpp, similar to single Vrk1^{Gt(RRR178)Byg} homozygotes

neoplasm

increased tumor incidence ( J:171525 )

reproductive system

seminiferous tubule degeneration ( J:171525 )

• marked loss/degeneration of the seminiferous tubular epithelium by 12 weeks of age

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age, similar to single Vrk1^{Gt(RRR178)Byg} homozygotes

early reproductive senescence ( J:171525 )

abnormal spermatogenesis ( J:171525 )

• progressive decline in spermatogenesis, starting at 8 weeks of age

• loss of spermatogenesis occurs earlier than in single Vrk1^{Gt(RRR178)Byg} homozygotes

female infertility ( J:171525 )

• females fail to produce litters or show any signs of pregnancy, similar to single Vrk1^{Gt(RRR178)Byg} female homozygotes

• however, copulatory plugs are observed

male infertility ( J:171525 )

• males fail to sire a litter after 69 days postpartum (dpp), despite continued presence of copulatory plugs

endocrine/exocrine glands

seminiferous tubule degeneration ( J:171525 )

• marked loss/degeneration of the seminiferous tubular epithelium by 12 weeks of age

decreased testis weight ( J:171525 )

• testis weight is significantly decreased at all ages examined between 6-8 and >12 weeks of age, similar to single Vrk1^{Gt(RRR178)Byg} homozygotes

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