All Phenotypes Cpa3<tm3(icre)Hrr> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Cpa3^tm3(icre)Hrr/Cpa3⁺	B6.129P2-Cpa3^tm3(icre)Hrr	MGI:5307246
ht2	Cpa3^tm3(icre)Hrr/Cpa3⁺	involves: 129P2/OlaHsd * BALB/c * C57BL/6	MGI:5307248
cn3	Cpa3^tm3(icre)Hrr/Cpa3⁺ Notch1^tm1Agt/Notch1^tm1Agt	involves: 129 * C57BL/6	MGI:3829970
cn4	Cpa3^tm3(icre)Hrr/Cpa3⁺ Gt(ROSA)26Sor^tm1Hjf/? Notch1^tm1Agt/Notch1^tm1Agt	involves: 129 * C57BL/6	MGI:3829973
cn5	Cpa3^tm3(icre)Hrr/Cpa3⁺ Fgfr1^tm1Upir/Fgfr1^tm1Upir Fgfr2^tm1Dor/Fgfr2^tm1Dor Tg(KRT5-cre)5132Jlj/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * C57BL/6J * DBA/2J	MGI:5642134

Allelic Composition	Cpa3^tm3(icre)Hrr/Cpa3⁺
Genetic Background	B6.129P2-Cpa3^tm3(icre)Hrr

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Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cpa3^tm3(icre)Hrr mutation (0 available); any Cpa3 mutation (30 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:178942 )

• Th2 immune response is not different from controls in mast cell-deficient animals

• IgM, IgG1, IgG2a, IgG2b, IgG3, IgA and IgE serum titers are not different from controls

• mice injected with K/Bxn serum in an antigen-induced arthritis model develop arthritis with rapid onset and similar severity relative to controls

• mice have a normal T cell-dependent antibody response to immunization with NP-chicken gamma globulin

decreased basophil cell number ( J:178942 )

• mice show reduced numbers of splenic basophils

absent mast cells ( J:178942 )

• mice exhibit complete absence of mast cells in the peritoneum, skin and other tissues that normally contain mast cells

• peritoneal and skin mast cell numbers are partially rescued on a Trp53-null background

abnormal inflammatory response ( J:178942 )

• mice show a complete lack of mast cells in the skin following repeated exposure to the inflammatory agent phorbol myristate acetate (PMA) whereas Kit mutants exhibit a 10-100-fold increase in mast cell numbers in the skin following exposure to PMA for 6 weeks

• after infection with helminthes, mice show no mucosal mast cell response in the intestine

hematopoietic system

hematopoietic system phenotype ( J:178942 )

N	• hematological parameters (counts of total white blood cells, red blood cells, platelets, hematocrit, and percentages of lymphocytes, monocytes, and neutrophils) are not affected in mutants

decreased basophil cell number ( J:178942 )

• mice show reduced numbers of splenic basophils

absent mast cells ( J:178942 )

• mice exhibit complete absence of mast cells in the peritoneum, skin and other tissues that normally contain mast cells

• peritoneal and skin mast cell numbers are partially rescued on a Trp53-null background

Allelic Composition	Cpa3^tm3(icre)Hrr/Cpa3⁺
Genetic Background	involves: 129P2/OlaHsd * BALB/c * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cpa3^tm3(icre)Hrr mutation (0 available); any Cpa3 mutation (30 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased susceptibility to type I hypersensitivity reaction ( J:178942 )

• mice are resistant to IgE-mediated anaphylaxis; animals do not display expected body temperature drop after antigen challenge

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

thymus hypoplasia ( J:143731 )

• thymus cellularity is reduced 65% compared to age-matched controls

abnormal T cell receptor beta chain V(D)J recombination ( J:143731 )

• some CD19^lo thymic B cells have recombined Tcrb Dbeta1-Jbeta1 gene segments

decreased double-negative T cell number ( J:143731 )

• the percentage of thymocytes in the DN stages of T cell development are reduced compared to controls

• the percentage of thymocytes in the DN1 stage is reduced by a third with most of the reduction occurring at the DN1a and DN1b sub-stages

• the percentage of thymocytes in the DN2-DN3 stages are reduced by about 50% compared to controls

decreased double-positive T cell number ( J:143731 )

• double positive thymocytes make up just 65% of total thymocyte population compared to 80% in controls

increased single-positive T cell number ( J:143731 )

• percentages of single-positive cells are increased in the thymus compared to controls

abnormal B cell morphology ( J:143731 )

• 10-fold more B cells are found in the thymus compared to controls

• these B cells consist of a major CD19^hi population and a minor CD19^lo population with similar populations found in the bone marrow

• the CD19^hi B cell population expresses cell surface markers similar to mature B2 B cells

• the CD19^loB cell population have cell surface markers of pro- and pre- B cells

• single cell genotyping reveals about 94% of the CD19^lo population has cre-mediated deletion of the floxed allele while only 26% of the CD19^hi allele had the flox allele deleted

abnormal B cell differentiation ( J:143731 )

• the presence of recombined Tcrb gene segments in the abnormal CD19^lo thymic B cells population suggests that the B cells differentiate from a DN1 T cell precursor

hematopoietic system

thymus hypoplasia ( J:143731 )

• thymus cellularity is reduced 65% compared to age-matched controls

abnormal T cell receptor beta chain V(D)J recombination ( J:143731 )

• some CD19^lo thymic B cells have recombined Tcrb Dbeta1-Jbeta1 gene segments

decreased double-negative T cell number ( J:143731 )

• the percentage of thymocytes in the DN stages of T cell development are reduced compared to controls

• the percentage of thymocytes in the DN1 stage is reduced by a third with most of the reduction occurring at the DN1a and DN1b sub-stages

• the percentage of thymocytes in the DN2-DN3 stages are reduced by about 50% compared to controls

decreased double-positive T cell number ( J:143731 )

• double positive thymocytes make up just 65% of total thymocyte population compared to 80% in controls

increased single-positive T cell number ( J:143731 )

• percentages of single-positive cells are increased in the thymus compared to controls

abnormal B cell morphology ( J:143731 )

• 10-fold more B cells are found in the thymus compared to controls

• these B cells consist of a major CD19^hi population and a minor CD19^lo population with similar populations found in the bone marrow

• the CD19^hi B cell population expresses cell surface markers similar to mature B2 B cells

• the CD19^loB cell population have cell surface markers of pro- and pre- B cells

• single cell genotyping reveals about 94% of the CD19^lo population has cre-mediated deletion of the floxed allele while only 26% of the CD19^hi allele had the flox allele deleted

abnormal B cell differentiation ( J:143731 )

• the presence of recombined Tcrb gene segments in the abnormal CD19^lo thymic B cells population suggests that the B cells differentiate from a DN1 T cell precursor

endocrine/exocrine glands

thymus hypoplasia ( J:143731 )

• thymus cellularity is reduced 65% compared to age-matched controls

Allelic Composition	Cpa3^tm3(icre)Hrr/Cpa3⁺ Gt(ROSA)26Sor^tm1Hjf/? Notch1^tm1Agt/Notch1^tm1Agt
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cpa3^tm3(icre)Hrr mutation (0 available); any Cpa3 mutation (30 available)
Gt(ROSA)26Sor^tm1Hjf mutation (5 available); any Gt(ROSA)26Sor mutation (942 available)
Notch1^tm1Agt mutation (0 available); any Notch1 mutation (115 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal dendritic cell differentiation ( J:143731 )

• cell-fate mapping experiments suggest that some thymic dendritic cells are derived from DN1 T cell precursors

abnormal T cell receptor beta chain V(D)J recombination ( J:143731 )

• recombined Tcrb Dbeta1-Jbeta1 gene segments are detectable in some thymic plasmacytoid dendritic cells and lymphoid dendritic cells

hematopoietic system

abnormal dendritic cell differentiation ( J:143731 )

• cell-fate mapping experiments suggest that some thymic dendritic cells are derived from DN1 T cell precursors

abnormal T cell receptor beta chain V(D)J recombination ( J:143731 )

• recombined Tcrb Dbeta1-Jbeta1 gene segments are detectable in some thymic plasmacytoid dendritic cells and lymphoid dendritic cells

cellular

abnormal dendritic cell differentiation ( J:143731 )

• cell-fate mapping experiments suggest that some thymic dendritic cells are derived from DN1 T cell precursors

Allelic Composition	Cpa3^tm3(icre)Hrr/Cpa3⁺ Fgfr1^tm1Upir/Fgfr1^tm1Upir Fgfr2^tm1Dor/Fgfr2^tm1Dor Tg(KRT5-cre)5132Jlj/0
Genetic Background	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * C57BL/6J * DBA/2J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cpa3^tm3(icre)Hrr mutation (0 available); any Cpa3 mutation (30 available)
Fgfr1^tm1Upir mutation (0 available); any Fgfr1 mutation (221 available)
Fgfr2^tm1Dor mutation (3 available); any Fgfr2 mutation (87 available)
Tg(KRT5-cre)5132Jlj mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

decreased body weight ( J:221184 )

integument

increased keratinocyte proliferation ( J:221184 )

impaired skin barrier function ( J:221184 )

• increase in transepidermal water loss, indicating an epidermal barrier defect

skin inflammation ( J:221184 )

progressive hair loss ( J:221184 )

acanthosis ( J:221184 )

hematopoietic system

absent mast cells ( J:221184 )

homeostasis/metabolism

impaired skin barrier function ( J:221184 )

• increase in transepidermal water loss, indicating an epidermal barrier defect

immune system

absent mast cells ( J:221184 )

skin inflammation ( J:221184 )

cellular

increased keratinocyte proliferation ( J:221184 )

reproductive system

female infertility ( J:221184 )

• all females are sterile

reduced male fertility ( J:221184 )

• most males are sterile

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