Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
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normal phenotype
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• mice are indistinguishable from wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
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cellular
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• mouse embryonic fibroblasts transfected with an adenovirus cre fail to produce micro RNAs and exhibit increased DNA damage that leads to premature senescence
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• mouse embryonic fibroblasts transfected with an adenovirus cre exhibit an increase in the number of cells in S phase
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• mouse embryonic fibroblasts transfected with an adenovirus cre cell proliferation is slowed compared to in wild-type cells
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• mouse embryonic fibroblasts transfected with an adenovirus cre exhibit premature senescence associated with increased DNA damage
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• mouse embryonic fibroblasts transfected with an adenovirus cre undergo less DNA replication than in wild-type cells
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homeostasis/metabolism
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
Tg(KRT5-cre/PGR)1Der mutation
(0 available)
Trp53tm1Brn mutation
(18 available);
any
Trp53 mutation
(232 available)
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mortality/aging
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• mean survival is 13 months, with none surviving beyond 18 months
• lethality before 1 year is unrelated to cancer as no tumors are seen until after 1 year of age
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integument
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• mice that survive beyond 1 year of age develop skin tumors
• less than 1/4 of mice develop more than a single tumor
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• mice form mostly basal cell carcinomas
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neoplasm
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• mice that survive beyond 1 year of age develop skin tumors
• less than 1/4 of mice develop more than a single tumor
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• mice form mostly basal cell carcinomas
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
Tg(KRT5-cre/PGR)1Der mutation
(0 available)
Trp53tm1Brn mutation
(18 available);
any
Trp53 mutation
(232 available)
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mortality/aging
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• mean survival is 9 months
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integument
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• loss of fur by the 5th week of age, with mice nearly devoid of fur and whiskers by 12 weeks of age
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• dysmorphic hair follicles with dyskertotic cells in the follicular epithelium
• regions of increased cellularity with many mitotic figures are seen in the interfollicular epidermal layer and above the basal layer
• large amount of DNA damage in the basal layer of follicular epithelium and in the interfollicular epidermis
• there is some evidence of attempts to form new follicles
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• at a time when control hair follicles are in the telogen phase of the hair cycles, mutants show anagen growth phase hairs with dysmorphic follicles, abnormal hair shafts, and dyskertotic cells in the follicular epithelium
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• pre-malignant basaloid proliferation is seen
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• increase in thickness of the epidermis with a mean thickness of 51.3 um compared to 24 um in controls
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• mice develop highly aggressive and numerous skin tumors starting at 7-8 months of age with all mice showing skin tumors by 12-15 months of age
• 41% of mice form multiple tumors
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• mice form moderately or poorly differentiated squamous cell carcinomas and nearly half of mutants develop poorly differentiated carcinomas
• poorly or undifferentiated carcinomas are highly invasive
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• the number of apoptotic cells in the epidermis is lower than in single conditional Dicer1 mutants
• increase in the numbers of mitotic cells in the epidermis, indicating increased proliferation
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neoplasm
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• mice develop highly aggressive and numerous skin tumors starting at 7-8 months of age with all mice showing skin tumors by 12-15 months of age
• 41% of mice form multiple tumors
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• mice form moderately or poorly differentiated squamous cell carcinomas and nearly half of mutants develop poorly differentiated carcinomas
• poorly or undifferentiated carcinomas are highly invasive
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
Trp53tm1Brd mutation
(5 available);
any
Trp53 mutation
(232 available)
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cellular
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• mouse embryonic fibroblasts treated with an adenovirus cre exhibit normal cellular senescence
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
Tg(CAG-cre/Esr1*)5Amc mutation
(9 available)
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limbs/digits/tail
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• at E14 to E16, tamoxifen treated mice exhibit increased cellular senescence in developing limbs compared to in wild-type mice
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
Tg(KRT5-cre/PGR)1Der mutation
(0 available)
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mortality/aging
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• mice die as early as 6 months of age, with a mean survival of 16 months and 80% dying by 20 months of age
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integument
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• at 2 to 3 months of age
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• mice begin to lose their fur and whiskers shortly after the 5th week of age, by 10 weeks of age, mice only retain small patches of fur and are devoid of any fur at 3 months of age
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• interfollicular epidermis exhibits an increase in cellularity
• large amount of DNA damage in the basal layer of follicular epithelium and in the interfollicular epidermis
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• hair follicles are debris-laden and mis-oriented and enlarged cells and apoptotic figures are seen surrounding the degraded follicle structure
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• mice begin to lose their whiskers shortly after the 5th week of age, by 10 weeks of age, mice show only a few misshapen whiskers, and mice are devoid of whiskers at 3 months of age
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• at 2 to 3 months of age, mice exhibit a roughening of the epidermis
(J:139257)
• cells are larger than in wild-type mice and display a senescence phenotype
(J:139257)
• mice exhibit a roughened epidermis at 3 months of age
(J:216813)
• interfollicular epidermis exhibits an increase in cellularity
(J:216813)
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• increase in thickness of the epidermis with a mean thickness of 42 um compared to 24 um in controls
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• increase in the numbers of apoptotic cells in the epidermis
• increase in the numbers of mitotic cells in the epidermis, indicating increased proliferation
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growth/size/body
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• several mutants exhibit mild cachexia before death
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neoplasm
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• no tumors are seen upon death
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm1Rdp mutation
(6 available);
any
Cdkn2a mutation
(62 available)
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
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cellular
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N |
• mouse embryonic fibroblasts treated with an adenovirus cre exhibit normal cellular senescence
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
Trp53tm1Brd mutation
(5 available);
any
Trp53 mutation
(232 available)
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdkn2atm1Rdp mutation
(6 available);
any
Cdkn2a mutation
(62 available)
Dicer1tm1Snj mutation
(0 available);
any
Dicer1 mutation
(94 available)
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Analysis Tools
