Phenotypes associated with this allele

• Allele Symbol: Kremen1^tm1Dgen
• Allele Name: targeted mutation 1, Deltagen
• Allele ID: MGI:3804430
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kremen1^tm1Dgen/Kremen1^tm1Dgen	either: B6.Cg-Kremen1^tm1Dgen or (involves: CD-1)	MGI:3805001
cx2	Dkk1^tm1Lmgd/Dkk1^+ Kremen1^tm1Dgen/Kremen1^tm1Dgen Kremen2^tm1Cni/Kremen2^tm1Cni	either: B6.Cg-Kremen1^tm1Dgen Kremen2^tm1Cni Dkk1^tm1Lmgd or (involves: 129S1/Sv * 129X1/SvJ * CD-1)	MGI:3805007
cx3	Kremen1^tm1Dgen/Kremen1^tm1Dgen Kremen2^tm1Cni/Kremen2^tm1Cni	either: B6.Cg-Kremen1^tm1Dgen Kremen2^tm1Cni or (involves: CD-1)	MGI:3805003

Genotype
MGI:3805001

hm1

• Allelic Composition: Kremen1^tm1Dgen/Kremen1^tm1Dgen
• Genetic Background: either: B6.Cg-Kremen1^tm1Dgen or (involves: CD-1)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:137914 )

• mice exhibit no obvious abnormalities

Genotype
MGI:3805007

cx2

• Allelic Composition: Dkk1^tm1Lmgd/Dkk1⁺; Kremen1^tm1Dgen/Kremen1^tm1Dgen; Kremen2^tm1Cni/Kremen2^tm1Cni
• Genetic Background: either: B6.Cg-Kremen1^tm1Dgen Kremen2^tm1Cni Dkk1^tm1Lmgd or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

skeleton

increased bone volume ( J:137914 )

• limb bone volume is increased compared to in wild-type mice but not compared to in Dkk1^tm1Lmgd heterozygotes

limbs/digits/tail

polydactyly ( J:137914 )

• mice have ectopic postaxial forelimb digits

• the frequency and size of ectopic digits is increase compared to in Kremen1^tm1Dgen Kremen2^tm1Cni homozygotes

• 40% of ectopic digits originate at the carpus like regular digits unlike in Kremen1^tm1Dgen Kremen2^tm1Cni homozygotes where ectopic digits originate from digit V

• however, unlike in Dkk1 null mice webbing of bone elements is not observed

Genotype
MGI:3805003

cx3

• Allelic Composition: Kremen1^tm1Dgen/Kremen1^tm1Dgen; Kremen2^tm1Cni/Kremen2^tm1Cni
• Genetic Background: either: B6.Cg-Kremen1^tm1Dgen Kremen2^tm1Cni or (involves: CD-1)

skeleton

abnormal long bone metaphysis morphology ( J:137914 )

• while trabecular thickness is normal, the number of trabeculae is increased and trabecular separation is decreased compared to in wild-type mice

increased bone volume ( J:137914 )

• limb bone volume is double that in wild-type mice

abnormal osteoblast morphology ( J:137914 )

• bone forming osteoblast surface and osteoid surface are increased compared to in wild-type mice

increased osteoblast cell number ( J:137914 )

increased bone mineralization ( J:137914 )

• mineral apposition rates are increased compared to in wild-type mice

increased bone ossification ( J:137914 )

• bone formation rates are increased compared to in wild-type mice

• however, bone absorption is normal

limbs/digits/tail

polydactyly ( J:137914 )

• mice have ectopic postaxial forelimb digits that are smaller or larger than digit V

• ectopic digits originate from digit V

reproductive system

reproductive system phenotype ( J:137914 )

N • contrary to results using anti-sense inhibition, mice exhibit normal litter sizes and normal implantation