Analysis Tools|
Allele Symbol Allele Name Allele ID |
Tg(CAG-EGFP/CETN2)3-4Jgg transgene insertion 3-4, Joseph G Gleeson MGI:3793421 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice exhibit normal body weights and do not develop hydrocephalus, unlike males carrying the Cetn2tm1.1Wbae allele
• mice are rescued from the OSN ciliary trafficking defect, as shown by normal ciliary localizations of two major transmembrane signaling proteins (ACIII and CNGA2), and a peripheral membrane-associated protein
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in P10 and P15 mice, only very few (about 3%) of basal bodies in photoreceptors are able to extend a transition zone; normal-length transitions zone extensions are absent, but some basal bodies carry short stunted extensions, suggesting aborted transition zone formation
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• Prom1, a key regulator of disk morphogenesis in the photoreceptors is distributed diffusely in the inner segment, suggesting disruption of disk morphogenesis
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• in P10 and P15 mice, only very few (about 3%) of basal bodies in photoreceptors are able to extend a transition zone; normal-length transitions zone extensions are absent, but some basal bodies carry short stunted extensions, suggesting aborted transition zone formation
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• Prom1, a key regulator of disk morphogenesis in the photoreceptors is distributed diffusely in the inner segment, suggesting disruption of disk morphogenesis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at P3-P4, when ependymal progenitor cells differentiate into multiciliated cells (MCCs), no DEUP1 foci are detected in the brain ventricular walls, indicating that deuterosomes are absent, unlike in wild-type ependymal cells
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• at P3-P4, when ependymal progenitor cells differentiate into MCCs, no deuterosomes are detected in the brain ventricular walls
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• live imaging of differentiating ependymal cells shows normal stepwise kinetics of centriole amplification in the absence of deuterosomes; however, the centriolar pathway is enhanced and the procentrioles present in the cytoplasm are organized into smaller groups that do not adopt a ring-shaped morphology
• in the absence of deuterosomes, more procentrioles form along the entire length of the parent centrioles (enabling growth of up to 18 procentrioles from a single parent centriole) while singlets or groups of 2 or 3 procentrioles are also noted in the vicinity of the parent centrioles and further away in the cytoplasm, unlike in control cells where procentrioles are predominantly nucleated from the proximal end of the parent centrioles
• as the amplification stage progresses, singlets or groups of procentrioles are released into the cytoplasm and are organized around the nuclear envelope in a configuration similar to that seen in control cells
• despite the increase in nucleation of procentrioles on the parent centrioles, incomplete rings of SAS6 are often seen around the circumference of parent centrioles and sites unoccupied by procentrioles are observed on the walls of the parent centrioles
• ependymal progenitor cells treated with centrinone (a PLK4 inhibitor) during the proliferation phase to deplete parent centrioles show normal centriole amplification; the final number of centrioles produced in cells with zero parent centrioles is similar to that in cells with two parent centrioles, and procentrioles still form in the absence of pre-existing centrioles and deuterosomes, as either single free-standing procentrioles or small groups of 2 or 3 procentrioles within a cloud of pericentriolar and fibrogranular material
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• very few cells from E9.0 embryos show centrioles (2% compared to 98% in wild-type embryos)
• in cultured ES cells almost half of all centrioles are abnormal, about one third have normal-like centrioles, and the rest have thread-like structures
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• very few cells from E9.0 embryos show centrioles (6% compared to 98% in wild-type embryos)
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• regions of cerebellum and ventricular zone show no increased levels of apoptosis or pyknotic nuclei compared to wild-type embryos
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• intracellular GFP-expressing aggregates are observed exclusively in the adult brain in specific regions (T.G.G. personal comm.)
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 01/06/2026 MGI 6.24 |
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