Phenotypes associated with this allele
Allelic Composition |
Gata1tm1Mym/Gata1+
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Genetic Background |
involves: 129P2/OlaHsd |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
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mortality/aging
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• more than half of females die within 7 months of birth
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• more than 50% of females die within 7 months of birth
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hematopoietic system
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• varying degrees of splenomegaly, with an accumulation of proerythroblasts and megakaryocytes
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• ectopic liver hematopoiesis
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• mutants with severe splenomegaly that die spontaneously exhibit a diminished number of megakaryocytes in the enlarged spleens, however mutants with moderate splenomegaly, exhibit massive accumulation of megakaryocytes in the spleen
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• massive accumulation of proerythroblasts in the spleen, resulting in almost no white pulp and only residual visible red pulp
• high numbers of proerythroblasts are seen in the peripheral blood of mice with severe anemia
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• only residual red pulp due to massive accumulation of proerythroblasts in the spleen
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• almost no white pulp due to massive accumulation of proerythroblasts in the spleen
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immune system
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• varying degrees of splenomegaly, with an accumulation of proerythroblasts and megakaryocytes
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• only residual red pulp due to massive accumulation of proerythroblasts in the spleen
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• almost no white pulp due to massive accumulation of proerythroblasts in the spleen
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growth/size/body
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• varying degrees of splenomegaly, with an accumulation of proerythroblasts and megakaryocytes
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Allelic Composition |
Gata1tm1Mym/Gata1+
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Genetic Background |
involves: 129P2/OlaHsd * BALB/c |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
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Variable phenotypes of Gata1tm1Mym/Gata1+ female embryos including anemia and reduced number of hematopoietic cells
hematopoietic system
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• varying degrees of impairment observed during embryonic and neonatal stages
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• some females exhibited severe anemia while others showed normal erytrhopoiesis, putatively due to random X-inactivation
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
Tg(Gata1*)#Mym mutation
(0 available)
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hematopoietic system
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• in both the erythroid and megakaryocytic lineages
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• at E13.5, mice exhibit anemia unlike Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• however, mice recover from anemia by E15.5
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• average ploidy is reduced compared to in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
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• at P0 and P5, the number of megakaryocytes in the liver is increased compared to in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• however, between P0 and P5 the number of megakaryocytes is normal in the spleen
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• at E12.5 and E15.5, mice exhibit hyperproliferative megakaryocyte progenitors compared with Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• cultured fetal livers from E15.5 and E18.5 mice produce more numerous smaller cells in a CFU-Mk assay compared to samples from fetal livers of control mice
• however, the numbers of CFU-Mk in E18.5 fetal livers is normal
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• at P0, abnormal red blood cells are found in blood smear including ones with nucleus and denatured cells unlike in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
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• at E13.5, erythroid differentiation as assessed by CD71 expression is severely disrupted compared to in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• however, erythroid differentiation is normal by E15.5
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• megathrombocytes and blast-like cells with prominent Golgi area and poorly developed cytoplasmic granulation are observed unlike in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
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immune system
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
Tg(Gata1*)#Mym mutation
(0 available)
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hematopoietic system
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• in both the erythroid and megakaryocytic lineages
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• at E13.5, mice exhibit anemia unlike Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• however, mice recover from anemia by E15.5
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• average ploidy is reduced compared to in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
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• at P0 and P5, the number of megakaryocytes in the liver is increased compared to in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• however, between P0 and P5 the number of megakaryocytes is normal in the spleen
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• at E12.5 and E15.5, mice exhibit hyperproliferative megakaryocyte progenitors compared with Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• cultured fetal livers from E15.5 and E18.5 mice produce more numerous smaller cells in a CFU-Mk assay compared to samples from fetal livers of control mice
• however, the numbers of CFU-Mk in E18.5 fetal livers is normal
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• at P0, abnormal red blood cells are found in blood smear including ones with nucleus and denatured cells unlike in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
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• at E13.5, erythroid differentiation as assessed by CD71 expression is severely disrupted compared to in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
• however, erythroid differentiation is normal by E15.5
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• megathrombocytes and blast-like cells with prominent Golgi area and poorly developed cytoplasmic granulation are observed unlike in Gata1tm1Mym/Gata1tm1Mym Tg(Gata1)#Mym control mice
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immune system
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
Tg(Gata1)#Mym mutation
(0 available)
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hematopoietic system
N |
• unlike Gata1tm1Mym homozygotes, mice do not exhibit anemia
• mice exhibit normal numbers of megakaryocytes at P5
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
Tg(Gata1)#Mym mutation
(0 available)
|
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hematopoietic system
N |
• unlike Gata1tm1Mym homozygotes, mice do not exhibit anemia
• mice exhibit normal numbers of megakaryocytes at P5
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
Tg(Gata1*V205G)1Mym mutation
(0 available)
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growth/size/body
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• E15.5 fetuses are sometimes small in size
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hematopoietic system
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• megakaryopoiesis is severerly affected
• analysis of in vitro colony formation from mononuclear ells indicates that hematopoietic progenitor formation is normal, however AChE+ cells of CFU-Megs are larger indicating dysregulation of megakaryopoiesis
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• megakaryocytes are larger in mutant fetuses than in wild-type
• megakaryocytes in the spleen are larger than in wild-type spleen
• megakaryocytes contain a large volume of cytoplasm with a paucity of cytoplasmic organelles
• demarcation membrane is underdeveloped in megakaryocytes
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• impaired megakaryocyte differentiation
• mutants exhibit dysregulated proliferation and impaired cytoplasmic maturation of megakaryocytes in adulthood
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• number of megakaryocytes is increased in E17.5 mutant livers and in hematopoietic tissues of adults
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• peripheral erythrocytes are morphologically abnormal
• in fetuses, circulation of nucleated erythroid cells is often observed whereas in wild-type mice, erythrocytes are uniformly enucleated
• enucleated erythroid cells are rarely seen in the fetal liver
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• mutants require longer time (11 days versus 7 days for wild-type mice) to recover from PHZ-induced anemia, indicating attenuated stress erythropoiesis
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• gross morphologic abnormalities of platelets are seen
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• proplatelet formation is impaired in the bone marrow cells of mutants
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• mutants exhibit severe thrombocytopenia without anemia
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• mutants exhibit an increased number of large mononuclear cells in the bone marrow
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immune system
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• proplatelet formation is impaired in the bone marrow cells of mutants
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• mutants exhibit an increased number of large mononuclear cells in the bone marrow
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integument
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• E15.5 fetuses appear pale
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cellular
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• impaired megakaryocyte differentiation
• mutants exhibit dysregulated proliferation and impaired cytoplasmic maturation of megakaryocytes in adulthood
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Allelic Composition |
Gata1tm1Mym/Y
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Genetic Background |
involves: 129P2/OlaHsd |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
|
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Allelic Composition |
Gata1tm1Mym/Y
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Genetic Background |
involves: 129P2/OlaHsd * BALB/c |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gata1tm1Mym mutation
(0 available);
any
Gata1 mutation
(44 available)
|
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Gata1tm1Mym/Y embryos are smaller and anemic
mortality/aging
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• embryonic lethality is first detected at E11.5 and no live males seen by E12.5
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hematopoietic system
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• impaired primitive erythropoiesis
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• decrease in the number of erythroid cells and CFU-E in livers
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embryo
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• yolk sacs contain almost no blood vessels at E9.5
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• impaired primitive erythropoiesis
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growth/size/body
liver/biliary system
cardiovascular system
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• yolk sacs contain almost no blood vessels at E9.5
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