Phenotypes associated with this allele

• Allele Symbol: Fen1⁺
• Allele Name: wild type
• Allele ID: MGI:2432996
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Fen1^tm1Bhsh/Fen1^+	involves: 129S1/Sv	MGI:3713690
ht2	Fen1^tm2.1Bhsh/Fen1^+	involves: 129S1/Sv	MGI:5694455
ht3	Fen1^tm1Rak/Fen1^+	involves: 129/Sv * C57BL/6 * SJL/J	MGI:3843880
ht4	Fen1^tm1Klng/Fen1^+	involves: C57BL/6	MGI:2670042
ht5	Fen1^tm3.1Bhsh/Fen1^+	Not Specified	MGI:5771898
cx6	Fen1^tm1Rak/Fen1^+ Apc^tm1Rak/Apc^+	involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * SJL/J	MGI:3843881

Genotype
MGI:3713690

ht1

• Allelic Composition: Fen1^tm1Bhsh/Fen1⁺
• Genetic Background: involves: 129S1/Sv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:121378 )

• mice are indistinguishable from wild-type

Genotype
MGI:5694455

ht2

• Allelic Composition: Fen1^tm2.1Bhsh/Fen1⁺
• Genetic Background: involves: 129S1/Sv

cellular

aneuploidy ( J:218627 )

• average % of near-tetraploid aneuploidy in heterozygous mutant MEFs cells is 9.6% relative to 6.6% for wild-type MEFs

decreased fibroblast proliferation ( J:218627 )

• under normal culture conditions, heterozygous mutant MEFs show mild-to-moderate defects in cell growth relative to wild-type MEFs

• upon treatment with camptothecin (CPT), the number of heterozygous mutant live cells is increased by only 1.7% per day, whereas the number of wild-type cells is increased by 10% per day

spontaneous chromosome breakage ( J:218627 )

• heterozygous mutant MEFs exhibit significantly more spontaneous chromosome breaks (1.0%) than wild-type MEFs (0.6%)

Genotype
MGI:3843880

ht3

• Allelic Composition: Fen1^tm1Rak/Fen1⁺
• Genetic Background: involves: 129/Sv * C57BL/6 * SJL/J

immune system

thymus atrophy ( J:77962 )

• heterozygotes are generally viable and fertile with a normal life span

• a small number show thymic atropy and become moribund at 5 or 11 months of age

neoplasm

increased B cell derived lymphoma incidence ( J:77962 )

• some mice found with non-Hodgkin's lymphoma of B cells

digestive/alimentary system

abnormal Paneth cell morphology ( J:77962 )

• one example found of Paneth cell hyperplasia in the small intestine

hematopoietic system

thymus atrophy ( J:77962 )

• heterozygotes are generally viable and fertile with a normal life span

• a small number show thymic atropy and become moribund at 5 or 11 months of age

endocrine/exocrine glands

abnormal Paneth cell morphology ( J:77962 )

• one example found of Paneth cell hyperplasia in the small intestine

thymus atrophy ( J:77962 )

• heterozygotes are generally viable and fertile with a normal life span

• a small number show thymic atropy and become moribund at 5 or 11 months of age

Genotype
MGI:2670042

ht4

• Allelic Composition: Fen1^tm1Klng/Fen1⁺
• Genetic Background: involves: C57BL/6

normal phenotype

no abnormal phenotype detected ( J:84556 )

• heterozygotes are viable, fertile and morphologically normal in terms of size and growth, with no detectable histological abnormalities at 12-15 months of age relative to wild-type littermates

Genotype
MGI:5771898

ht5

• Allelic Composition: Fen1^tm3.1Bhsh/Fen1⁺
• Genetic Background: Not Specified

cellular

increased sensitivity to induced cell death ( J:231218 )

• primary MEFs exhibit significantly decreased cell survival following exposure to mitomycin C

increased cellular sensitivity to methylmethanesulfonate induced cell death ( J:231218 )

• primary MEFs exhibit significantly decreased cell survival following exposure to methylmethanesulfonate

chromosomal instability ( J:231218 )

• primary MEFs show ~12-fold higher spontaneous mutation rate than wild-type MEFs, as determined by an Hprt mutant assay

hematopoietic system

enlarged spleen ( J:231218 )

extramedullary hematopoiesis ( J:231218 )

• mice develop significant extrameduallry hematopoiesis

increased plasma cell number ( J:231218 )

• in 35% cases, large cluster or sheet-like plasma cells are seen in the interfollicular region

immune system

enlarged spleen ( J:231218 )

increased plasma cell number ( J:231218 )

• in 35% cases, large cluster or sheet-like plasma cells are seen in the interfollicular region

lymphoid hyperplasia ( J:231218 )

• mice develop a lymphoproliferative phenotype, characterized by marked follicular and interfollicular hyperplasia

increased susceptibility to autoimmune disorder ( J:231218 )

• mice develop autoimmunity and a lymphoproliferative disorder by 9-12 months of age

increased anti-nuclear antigen antibody level ( J:231218 )

• at 9-12 months of age, the serum anti-nuclear antigen antibody levels are 1.12 +/- 0.8 unit/ul relative to 0.35 +/- 0.3 unit/ul in wild-type mice

• CLIFT analysis revealed that antibodies to nuclear antigens present in sera are against dsDNA

increased anti-double stranded DNA antibody level ( J:231218 )

• at 9-12 months of age, the serum levels of anti-double stranded DNA antibodies are significantly higher than in wild-type controls

• DNA-IgG complex abundance is 2-3-fold higher than that in wild-type sera

chronic inflammation ( J:231218 )

• at 9-12 months of age, 43% of mice develop chronic lung inflammation relative to 19% of age-matched wild-type mice

• at >6 months of age, a significant number of mice develop chronic subcutaneous inflammation around the external urogenital area

glomerulonephritis ( J:231218 )

• mice show deposition of IgG-C3 immune complexes onto the glomeruli and capillary walls of the kidneys

lung inflammation ( J:231218 )

• at 9-12 months of age, 43% of mice develop chronic lung inflammation relative to 19% of age-matched wild-type mice

• deposition of IgG-C3 immune complex onto cells in the lungs

interstitial pneumonia ( J:231218 )

• mice develop pulmonary interstitial inflammation characterized by pneumonocyte hyperplasia and accumulation of inflammatory cells, including significant lymphoplasmacytic infiltrate

neoplasm

increased lung adenoma incidence ( J:231218 )

• at 14-20 months of age, 36.7% of mice developed lung adenoma relative to 17.6% of age-matched wild-type mice

• all mice had only one tumor, similar to affected wild-type mice

renal/urinary system

glomerulonephritis ( J:231218 )

• mice show deposition of IgG-C3 immune complexes onto the glomeruli and capillary walls of the kidneys

renal glomerular immunoglobulin deposits ( J:231218 )

respiratory system

lung inflammation ( J:231218 )

• at 9-12 months of age, 43% of mice develop chronic lung inflammation relative to 19% of age-matched wild-type mice

• deposition of IgG-C3 immune complex onto cells in the lungs

interstitial pneumonia ( J:231218 )

• mice develop pulmonary interstitial inflammation characterized by pneumonocyte hyperplasia and accumulation of inflammatory cells, including significant lymphoplasmacytic infiltrate

increased lung adenoma incidence ( J:231218 )

• at 14-20 months of age, 36.7% of mice developed lung adenoma relative to 17.6% of age-matched wild-type mice

• all mice had only one tumor, similar to affected wild-type mice

growth/size/body

enlarged spleen ( J:231218 )

Genotype
MGI:3843881

cx6

• Allelic Composition: Fen1^tm1Rak/Fen1⁺; Apc^tm1Rak/Apc⁺
• Genetic Background: involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * SJL/J

mortality/aging

premature death ( J:77962 )

• median survival of 9 months as compared to 13 months for Apc^tm1Rak single heterozygotes

neoplasm

increased gastrointestinal tumor incidence ( J:77962 )

• 100% develop gastrointestinal tumors by 1 year of age

increased malignant tumor incidence ( J:77962 )

• incidence of malignant tumors is higher than in Apc^tm1Rak single heterozygotes

cellular

abnormal cell physiology ( J:77962 )

• extensive microsatellite instability in tumor DNA

digestive/alimentary system

increased gastrointestinal tumor incidence ( J:77962 )

• 100% develop gastrointestinal tumors by 1 year of age