Fen1^tm3.1Bhsh
Targeted Allele Detail

Summary

• Symbol: Fen1^tm3.1Bhsh
• Name: flap structure specific endonuclease 1; targeted mutation 3.1, Binghui Shen
• MGI ID: MGI:5767323
• Synonyms: E160D, Fen1^ED
• Gene: Fen1 Location: Chr19:10176496-10181533 bp, - strand Genetic Position: Chr19, 6.54 cM
• Alliance: Fen1^tm3.1Bhsh page

(a) Accumulation of apoptotic nuclei in Fen1^tm3.1Bhsh/Fen1^tm3.1Bhsh tissues

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231218
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted Mutations: Insertion, Single point mutation Mutation details: The coding sequence was replaced with a floxed neomycin resistance cassette and a modified coding sequence in which a G to T point mutation results in the amino acid substitution of aspartic acid for glutamic acid at position 160 (E160D). Cre-mediated recombination removed the selection cassette. The E160D mutation abolishes >90% of the 5' exonuclease (EXO) and gap-dependent endonuclease (GEN) activities but retains the flap-specific endonuclease (FEN) protein activity, representing mutations identified in human cancers. Western blot analysis revealed that protein expression is unaffected in MEF lysates from heterozygous and homozygous mutant mice. (J:231218)

Generation of the Fen1^tm3.1Bhsh allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Fen1^tm3.1Bhsh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fen1 Mutation: 31 strains or lines available

References

• Original: J:231218 Zheng L, et al., Fen1 mutations result in autoimmunity, chronic inflammation and cancers. Nat Med. 2007 Jul;13(7):812-9
• All: 3 reference(s)