Phenotypes associated with this allele

• Allele Symbol: Gnao1⁺
• Allele Name: wild type
• Allele ID: MGI:2430743
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Gnao1^tm2.1Rneu/Gnao1^+	129S-Gnao1^tm2.1Rneu	MGI:5620504
ht2	Gnao1^tm2Rneu/Gnao1^+	B6.129S1-Gnao1^tm2Rneu	MGI:6295359
ht3	Gnao1^tm2.1Rneu/Gnao1^+	B6.129S-Gnao1^tm2.1Rneu	MGI:5620503
ht4	Gnao1^em2Rneu/Gnao1^+	C57BL/6J-Gnao1^em2Rneu	MGI:6513140
ht5	Gnao1^tm2.1Rneu/Gnao1^+	(C57BL/6J x 129S1/SvImJ)F1	MGI:5620505
ht6	Gnao1^tm1b(EUCOMM)Hmgu/Gnao1^+	C57BL/6N-Gnao1^tm1b(EUCOMM)Hmgu/H	MGI:5757180
ht7	Gnao1^tm2Rneu/Gnao1^+	involves: 129S1/Sv * C57BL/6J	MGI:5508325
ht8	Gnao1^tm1Ejne/Gnao1^+	involves: 129S4/SvJae	MGI:5620506
ht9	Gnao1^em1Rneu/Gnao1^+	involves: C57BL/6J * C57BL/6NCrl	MGI:6295357

Genotype
MGI:5620504

ht1

• Allelic Composition: Gnao1^tm2.1Rneu/Gnao1⁺
• Genetic Background: 129S-Gnao1^tm2.1Rneu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:216850 )

N • Background Sensitivity: 100% of mice on the 129S1/SvImJ background survive more than 1 year compared to premature lethality on a C57BL/6J background

behavior/neurological

behavior/neurological phenotype ( J:216850 )

♀N • Background Sensitivity: female mice show no difference in susceptibility to pentylenetetrazol (PTZ) kindling compared to faster sensitization on a C57BL/6J background

Genotype
MGI:6295359

ht2

• Allelic Composition: Gnao1^tm2Rneu/Gnao1⁺
• Genetic Background: B6.129S1-Gnao1^tm2Rneu

behavior/neurological

decreased exploration in new environment ( J:271137 )

• both males and females show reduced activity in the first 10 min of the open field arena indicating decreased activity in a novel environment

impaired coordination ( J:271137 )

♀ • females exhibit a reduced retention time on the accelerating rotarod, while males are unaffected

♀ • however, mice do not show a difference in learning rate on the rotarod

decreased grip strength ( J:271137 )

♀ • females, but not males, show reduced forepaw grip strength

abnormal gait ( J:271137 )

• females exhibit abnormal gait with reduced stride length and increased paw angle variability while males only show increased paw angle variability

short stride length ( J:271137 )

♀ • females show reduced stride length

decreased locomotor activity ( J:271137 )

• mice exhibit reduced activity in the open field arena, with both males and females showing reduced activity in the first 10 minutes (novel environment), and females, but not males, showing reduced activity in the next 10-30 min designated as sustained activity

• both males and females show reduced total activity

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
developmental and epileptic encephalopathy 17		DOID:0080450	OMIM:615473	J:271137

Genotype
MGI:5620503

ht3

• Allelic Composition: Gnao1^tm2.1Rneu/Gnao1⁺
• Genetic Background: B6.129S-Gnao1^tm2.1Rneu

mortality/aging

premature death ( J:216850 )

• mice that survive to weaning exhibit lethality starting at around 80 days with about 50% dying by 25 weeks of age

• the 50% that survive to 25 weeks show little further premature lethality

postnatal lethality, incomplete penetrance ( J:216850 )

• Background Sensitivity: premature lethality occurs after E18.5 but before 2 weeks of age on the C57BL/6J background but not on a 129S1/SvImJ background

behavior/neurological

abnormal kindling response ( J:216850 )

• Background Sensitivity: mice exhibit faster sensitization to pentylenetetrazol (PTZ) kindling than controls on the C57BL/6J background but not on a 129S1/SvImJ background

nervous system

abnormal kindling response ( J:216850 )

• Background Sensitivity: mice exhibit faster sensitization to pentylenetetrazol (PTZ) kindling than controls on the C57BL/6J background but not on a 129S1/SvImJ background

abnormal interictal brain wave pattern ( J:216850 )

♀ • 24-week old female mice exhibit elevated frequency of interictal epileptiform discharges, which occur more frequently during sleep than during wakefulness

♀ • interictal epileptiform discharges frequently occur in runs of 1-3 Hz, but none meet criteria for seizures due to the short duration of discharges of less than 10 seconds

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
developmental and epileptic encephalopathy 17		DOID:0080450	OMIM:615473	J:216850

Genotype
MGI:6513140

ht4

• Allelic Composition: Gnao1^em2Rneu/Gnao1⁺
• Genetic Background: C57BL/6J-Gnao1^em2Rneu

behavior/neurological

behavior/neurological phenotype ( J:303229 )

N • mice exhibit no overt postural or movement abnormalities or seizures under normal housing conditions

N • mice do not show increased susceptibility to PTZ kindling-induced seizures

abnormal response to new environment ( J:303229 )

♀ • females, but not males, show increased activity in the first 10 minutes (novelty period) in the open field arena

increased thigmotaxis ( J:303229 )

• mice display reduced time in the center of the open field arena, possibly indicating anxiety-like behavior

short stride length ( J:303229 )

♂ • males show a modestly reduced stride length

♂ • however, no difference in paw angle variability is seen in either males or females

hyperactivity ( J:303229 )

• mice show increased activity in the open field arena in the sustained activity period

• however, mice show no differences on the rotarod or grip strength test

• mice treated with risperidone, an atypical antipsychotic drug, show attenuation of hyperactivity; risperidone also reduces activity of wild-type mice

impaired exercise endurance ( J:303229 )

♀ • females show a reduced maximum run speed on the treadmill

homeostasis/metabolism

impaired exercise endurance ( J:303229 )

♀ • females show a reduced maximum run speed on the treadmill

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
neurodevelopmental disorder with involuntary movements		DOID:0112276	OMIM:617493	J:303229

Genotype
MGI:5620505

ht5

• Allelic Composition: Gnao1^tm2.1Rneu/Gnao1⁺
• Genetic Background: (C57BL/6J x 129S1/SvImJ)F1

mortality/aging

mortality/aging ( J:216850 )

N • Background Sensitivity: no deaths are seen up to 47 weeks of age compared to premature lethality on a congenic C57BL/6J background

behavior/neurological

behavior/neurological phenotype ( J:216850 )

♀N • Background Sensitivity: female mice show no difference in susceptibility to pentylenetetrazol (PTZ) kindling compared to faster sensitization on a congenic C57BL/6J background

Genotype
MGI:5757180

ht6

• Allelic Composition: Gnao1^{tm1b(EUCOMM)Hmgu}/Gnao1⁺
• Genetic Background: C57BL/6N-Gnao1^{tm1b(EUCOMM)Hmgu}/H
• Cell Lines: HEPD0618_2_G05

Data Sources

IMPC Data for Gnao1

behavior/neurological

decreased anxiety-related response ( J:211773 )

IMPC - HAR

impaired righting response ( J:211773 )

♀

IMPC - HAR

hyperactivity ( J:211773 )

IMPC - HAR

hematopoietic system

decreased eosinophil cell number ( J:211773 )

♀

IMPC - HAR

decreased erythrocyte cell number ( J:211773 )

♂

IMPC - HAR

increased mean corpuscular hemoglobin ( J:211773 )

♂

IMPC - HAR

decreased red blood cell distribution width ( J:211773 )

♀

IMPC - HAR

homeostasis/metabolism

increased circulating creatinine level ( J:211773 )

IMPC - HAR

decreased fasting circulating glucose level ( J:211773 )

♂

IMPC - HAR

increased circulating triglyceride level ( J:211773 )

♂

IMPC - HAR

decreased circulating iron level ( J:211773 )

IMPC - HAR

increased circulating amylase level ( J:211773 )

♂

IMPC - HAR

decreased circulating serum albumin level ( J:211773 )

♂

IMPC - HAR

increased blood urea nitrogen level ( J:211773 )

♂

IMPC - HAR

decreased respiratory quotient ( J:211773 )

♂

IMPC - HAR

immune system

decreased eosinophil cell number ( J:211773 )

♀

IMPC - HAR

skeleton

abnormal bone structure ( J:211773 )

♀

IMPC - HAR

decreased bone mineral content ( J:211773 )

♀

IMPC - HAR

Genotype
MGI:5508325

ht7

• Allelic Composition: Gnao1^tm2Rneu/Gnao1⁺
• Genetic Background: involves: 129S1/Sv * C57BL/6J

behavior/neurological

abnormal spike wave discharge ( J:197965 )

• mice treated with epinephrine exhibit enhanced reduction in burst frequency of induced epileptiform activity compared with control mice

nervous system

abnormal spike wave discharge ( J:197965 )

• mice treated with epinephrine exhibit enhanced reduction in burst frequency of induced epileptiform activity compared with control mice

Genotype
MGI:5620506

ht8

• Allelic Composition: Gnao1^tm1Ejne/Gnao1⁺
• Genetic Background: involves: 129S4/SvJae

behavior/neurological

behavior/neurological phenotype ( J:216850 )

N • mice do not show enhancement in sensitivity to pentylenetetrazol (PTZ) kindling

Genotype
MGI:6295357

ht9

• Allelic Composition: Gnao1^em1Rneu/Gnao1⁺
• Genetic Background: involves: C57BL/6J * C57BL/6NCrl

mortality/aging

premature death ( J:271137 )

• 2 of 33 mice die spontaneously around 5-7 weeks of age

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:271137 )

• a significant number of mice of both sexes fail to run when the belt speed exceeds 22 cm/s

impaired coordination ( J:271137 )

♂ • males exhibit a reduced retention time on the accelerating rotarod, while females are unaffected

♂ • however, mice do not show a difference in learning rate on the rotarod and mice exhibit normal performance in the open field arena

decreased grip strength ( J:271137 )

• both males and females exhibit a decrease in forepaw grip strength

abnormal gait ( J:271137 )

♂ • males exhibit abnormal gait, with males, but not females, showing increased paw angle variability and reduced stride length

short stride length ( J:271137 )

♂ • seen in males but not females

increased kindling response ( J:271137 )

♂ • males show increased sensitivity to pentylenetetrazole (PTZ) kindling

♂ • however, no spontaneous seizures are seen

nervous system

increased kindling response ( J:271137 )

♂ • males show increased sensitivity to pentylenetetrazole (PTZ) kindling

♂ • however, no spontaneous seizures are seen

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
developmental and epileptic encephalopathy 17		DOID:0080450	OMIM:615473	J:271137