Gnao1^tm2Rneu
Targeted Allele Detail

Summary

• Symbol: Gnao1^tm2Rneu
• Name: guanine nucleotide binding protein, alpha O; targeted mutation 1, Richard R Neubig
• MGI ID: MGI:5508226
• Synonyms: Galphao^G184S
• Gene: Gnao1 Location: Chr8:94536781-94696016 bp, + strand Genetic Position: Chr8, 45.94 cM
• Alliance: Gnao1^tm2Rneu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197965
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 5 was replaced with a modified exon 5 that contains nculeotide substitutions (GGC to AGT) that result in the amino acid substitution of serine for glycine at position 184 (G184S). This mutation blocks RGS binding. A floxed neo cassette was inserted between the modified exon 5 and exon 6. (J:197965)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnao1 Mutation: 29 strains or lines available

References

• Original: J:197965 Goldenstein BL, et al., Regulator of G protein signaling protein suppression of Galphao protein-mediated alpha2A adrenergic receptor inhibition of mouse hippocampal CA3 epileptiform activity. Mol Pharmacol. 2009 May;75(5):1222-30
• All: 6 reference(s)