Phenotypes associated with this allele

• Allele Symbol: Scd1^tm1Ntam
• Allele Name: targeted mutation 1, James M Ntambi
• Allele ID: MGI:2388371
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Scd1^tm1Ntam/Scd1^tm1Ntam	involves: 129S6/SvEvTac	MGI:2667734
hm2	Scd1^tm1Ntam/Scd1^tm1Ntam	involves: C57BL/6	MGI:3773312
cx3	Scd1^tm1Ntam/Scd1^tm1Ntam Sirt3^tm1.1Fwa/Sirt3^tm1.1Fwa	involves: 129S6/SvEvTac * C57BL/6	MGI:5311146

Genotype
MGI:2667734

hm1

• Allelic Composition: Scd1^tm1Ntam/Scd1^tm1Ntam
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased white adipose tissue amount ( J:78607 )

• especially reproductive and subcutaneous fat pads, both on high fat and normal chow diet

behavior/neurological

polyphagia ( J:78607 )

• consumed 25% more food than wild-type littermate controls

homeostasis/metabolism

decreased circulating insulin level ( J:78607 )

• in fasting state, compared to wild-type littermate controls

increased circulating ketone body level ( J:78607 )

increased oxygen consumption ( J:78607 )

improved glucose tolerance ( J:78607 )

• vs. wild-type littermate controls

abnormal cholesterol homeostasis ( J:71350 )

decreased unsaturated fatty acids level ( J:71350 )

• noted in liver, white adipose tissue, skin, eyelid

increased saturated fatty acids level ( J:71350 )

• noted in liver, white adipose tissue, skin, eyelid

vision/eye

Meibomian gland atrophy ( J:71350 )

narrow eye opening ( J:71350 )

• evident at 3 - 4weeks, and progressively more severe with age

integument

Meibomian gland atrophy ( J:71350 )

sebaceous gland atrophy ( J:71350 )

progressive hair loss ( J:71350 )

• evident at 3-4 weeks, progressively severe with age

endocrine/exocrine glands

Meibomian gland atrophy ( J:71350 )

sebaceous gland atrophy ( J:71350 )

Genotype
MGI:3773312

hm2

• Allelic Composition: Scd1^tm1Ntam/Scd1^tm1Ntam
• Genetic Background: involves: C57BL/6

growth/size/body

decreased susceptibility to diet-induced obesity ( J:130441 )

• resistant to both high fat and high carbohydrate diet-induced obesity

adipose tissue

decreased white adipose tissue amount ( J:130441 )

• on a high fat or high carbohydrate diet WAT weights are 80% and 75% lower, respectively, compared to diet-matched wild-type mice

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:130441 )

• resistant to both high fat and high carbohydrate diet-induced obesity

decreased circulating triglyceride level ( J:130441 )

• on a high fat or high carbohydrate diet, but not on a normal chow diet

abnormal glucose homeostasis ( J:130441 )

decreased circulating glucose level ( J:130441 )

• reduced fasting plasma glucose level on a normal chow, high fat or high carbohydrate diet compared to diet-matched wild-type mice

improved glucose tolerance ( J:130441 )

• enhanced tolerance is seen in mice on a normal chow, high fat or high carbohydrate diet compared to diet-matched wild-type mice

increased insulin sensitivity ( J:130441 )

• enhanced sensitivity is seen in mice on a normal chow, high fat or high carbohydrate diet compared to diet-matched wild-type mice

decreased liver triglyceride level ( J:130441 )

• decrease in palmitate and linoleate content in the liver of mice on a normal chow, high fat or high carbohydrate diet

abnormal lipid metabolism ( J:345295 )

♂ • after induction of myocardial infarction (MI), mice show suppression of AMPK phosphorylation in the infarcted heart, unlike wild-type controls

liver/biliary system

decreased liver triglyceride level ( J:130441 )

• decrease in palmitate and linoleate content in the liver of mice on a normal chow, high fat or high carbohydrate diet

decreased susceptibility to diet-induced hepatic steatosis ( J:130441 )

• protected from both high fat and high carbohydrate diet-induced fatty liver development

behavior/neurological

abnormal eating behavior ( J:130441 )

• increased in mice on a normal chow, high fat or high carbohydrate diet compared to diet-matched wild-type mice

cardiovascular system

abnormal physiological neovascularization ( J:345295 )

♂ • after induction of myocardial infarction (MI) by left coronary artery ligation, mice exhibit a decrease in VEGF-A protein levels in the myocardium and plasma and show lower serum levels of proangiogenic cytokines (IL1-beta, TNF-alpha, MCP-1, and Rantes) than sham controls

decreased angiogenesis ( J:345295 )

♂ • in an ex vivo aortic ring assay, aortic explants collected from mice either fed a chow diet or a high-fat (HF) diet for 6 weeks are unable to sprout capillary structures in a collagen matrix; in comparison, explants from chow-fed wild-type mice exhibit a higher angiogenic potential than HF-fed wild-type mice

Genotype
MGI:5311146

cx3

• Allelic Composition: Scd1^tm1Ntam/Scd1^tm1Ntam; Sirt3^tm1.1Fwa/Sirt3^tm1.1Fwa
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

homeostasis/metabolism

increased insulin sensitivity ( J:178653 )

• double mutants fed a high-fat diet exhibit improved insulin sensitivity compared to single Sirt3 homozygotes

decreased liver triglyceride level ( J:178653 )

• double mutants fed a high-fat diet exhibit reduced hepatic triglycerides when compared to single Sirt3 homozygotes

• however, mutants do not exhibit any differences from wild-type mice in hepatic lipids when fed a standard diet

liver/biliary system

decreased liver triglyceride level ( J:178653 )

• double mutants fed a high-fat diet exhibit reduced hepatic triglycerides when compared to single Sirt3 homozygotes

• however, mutants do not exhibit any differences from wild-type mice in hepatic lipids when fed a standard diet