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Allele Symbol Allele Name Allele ID |
Vav1tm1Tyb targeted mutation 1, Victor L J Tybulewicz MGI:2387838 |
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| Summary |
13 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• deletion of CD4+ cells expressing Vbeta3, 5, and 11 is less than in wild-type mice suggesting impaired negative selection
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• deletion of CD4+ cells expressing Vbeta3, 5, and 11 is less than in wild-type mice suggesting impaired negative selection
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• large decrease of single "+" cells
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• reduced ability of CD8+ cells to form conjugates with antigen presenting cells
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• large decrease of single "+" cells
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• reduced ability of CD8+ cells to form conjugates with antigen presenting cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• when stimulated with anti-CD3 antibodies or engagement of CD3 and CD28 receptors, T cell proliferation is impaired compared to in similarly treated wild-type cells
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• Staphylococcal enterotoxin B-induced deletion of single positive T cells is less than in wild-type mice
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• when stimulate by anti-CD3 and anti-CD3 and anti-CD28 antibodies, CD4+ T cells exhibit reduced blast population compared with similarly treated wild-type cells
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• when stimulated with anti-CD3 antibodies or engagement of CD3 and CD28 receptors, T cell proliferation is impaired compared to in similarly treated wild-type cells
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• T cells stimulated with anti-CD3 and anti-CD28 antibodies produce less IL2 than similarly treated wild-type or Rasgrf2tm1Esn cells
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• T cells stimulated with anti-CD3 or anti-CD3 and anti-CD28 antibodies produce less TNF-alpha than similarly treated wild-type or Rasgrf2tm1Esn cells
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• Staphylococcal enterotoxin B-induced deletion of single positive T cells is less than in wild-type mice
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• when stimulate by anti-CD3 and anti-CD3 and anti-CD28 antibodies, CD4+ T cells exhibit reduced blast population compared with similarly treated wild-type cells
|
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• when stimulated with anti-CD3 antibodies or engagement of CD3 and CD28 receptors, T cell proliferation is impaired compared to in similarly treated wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• about a 50% reduction in cell number
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• while the overall absolute number of double negative cells is similar to wild-type as are the numbers of early stage double negative cell subpopulation, the number of CD44-CD25+ cells is increased about 2-fold
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• reduced about 2-fold
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• small decrease in mature IgMloIgDhi B cells
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• about a 7- to 10-fold reduction in the number of single positive cells is seen in the thymus and in peripheral T cell populations in the spleen and lymph nodes
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• greatly reduced T cell receptor-induced calcium fluxes
(J:79323)
• calcium fluxes induced by anti-CD3 antibody cross-linking are diminished in T cells
(J:86654)
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• T cells show a partial defect in proliferative responses to stimulation with anti-CD3 with or without anti-CD28 antibodies
• ability of T cells to generate blasts when stimulated is reduced by 3- to 4-fold
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• production of IFN-gamma by T cells in response to TCR stimulation is reduced
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• production of IL-2 by T cells in response to TCR stimulation is reduced
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• about a 50% reduction in cell number
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• while the overall absolute number of double negative cells is similar to wild-type as are the numbers of early stage double negative cell subpopulation, the number of CD44-CD25+ cells is increased about 2-fold
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• reduced about 2-fold
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• small decrease in mature IgMloIgDhi B cells
|
|
• about a 7- to 10-fold reduction in the number of single positive cells is seen in the thymus and in peripheral T cell populations in the spleen and lymph nodes
|
|
• greatly reduced T cell receptor-induced calcium fluxes
(J:79323)
• calcium fluxes induced by anti-CD3 antibody cross-linking are diminished in T cells
(J:86654)
|
|
• T cells show a partial defect in proliferative responses to stimulation with anti-CD3 with or without anti-CD28 antibodies
• ability of T cells to generate blasts when stimulated is reduced by 3- to 4-fold
|
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• about a 50% reduction in cell number
|
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• T cells show a partial defect in proliferative responses to stimulation with anti-CD3 with or without anti-CD28 antibodies
• ability of T cells to generate blasts when stimulated is reduced by 3- to 4-fold
|
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• significant reduction in CD4+ cell numbers
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• significant reduction in CD5 and CD2 expression and T cell receptor-induced calcium fluxes
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• reduced CD4+ and CD8+ cell proliferation in response to anti-CD3 alone or anti-CD3 plus anti-CD28
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• significant reduction in CD4+ cell numbers
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• significant reduction in CD5 and CD2 expression and T cell receptor-induced calcium fluxes
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• reduced CD4+ and CD8+ cell proliferation in response to anti-CD3 alone or anti-CD3 plus anti-CD28
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• reduced CD4+ and CD8+ cell proliferation in response to anti-CD3 alone or anti-CD3 plus anti-CD28
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• deletion of CD4+ cells expressing Vbeta5, 11, and 12 is less than in wild-type mice suggesting impaired negative selection
|
|
• deletion of CD4+ cells expressing Vbeta5, 11, and 12 is less than in wild-type mice suggesting impaired negative selection
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• the number of IgMloIgDhi B cells is reduced in the lymph nodes compared to in wild-type mice
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• the number of IgMhiIgDlo B cells is reduced in the spleen compared to in wild-type mice
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• reduced 50% in the bone marrow and one-third in the spleen
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• the number of re-circulating follicular B cells is reduced 80% to 90% compared to in wild-type mice
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• BCR-stimulated B cells exhibit impaired calcium response compared to in similarly treated wild-type mice
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• in response to BCR stimulation
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• the number of IgMloIgDhi B cells is reduced in the lymph nodes compared to in wild-type mice
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• the number of IgMhiIgDlo B cells is reduced in the spleen compared to in wild-type mice
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• reduced 50% in the bone marrow and one-third in the spleen
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• the number of re-circulating follicular B cells is reduced 80% to 90% compared to in wild-type mice
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• BCR-stimulated B cells exhibit impaired calcium response compared to in similarly treated wild-type mice
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• in response to BCR stimulation
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• in response to BCR stimulation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• introduction of the Cblbtm1Pngr mutation into a Wastm1Sbs/Vav1tm1Tyb double null background fails to restore T cell responses and receptor clustering, indicating that WAS protein is critical for deregulated proliferation and membrane receptor reorganization of Cblbtm1Pngr mutant T cells
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• introduction of the Cblbtm1Pngr mutation into a Wastm1Sbs/Vav1tm1Tyb double null background fails to restore T cell responses and receptor clustering, indicating that WAS protein is critical for deregulated proliferation and membrane receptor reorganization of Cblbtm1Pngr mutant T cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• B cells show reduced proliferation in response to Ig receptor stimulation
|
|
• ability of T cells to generate blasts when stimulated is reduced by 3- to 4-fold, indicating a proliferation defect
|
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• 2- to 3-fold reduction in mature IgMloIgDhi B cells
|
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• few peripheral T cells
|
|
• production of IFN-gamma by T cells in response to TCR stimulation is reduced
|
|
• production of IL-2 by T cells in response to TCR stimulation is reduced
|
|
• B cells show reduced proliferation in response to Ig receptor stimulation
|
|
• ability of T cells to generate blasts when stimulated is reduced by 3- to 4-fold, indicating a proliferation defect
|
|
• 2- to 3-fold reduction in mature IgMloIgDhi B cells
|
|
• few peripheral T cells
|
|
• B cells show reduced proliferation in response to Ig receptor stimulation
|
|
• ability of T cells to generate blasts when stimulated is reduced by 3- to 4-fold, indicating a proliferation defect
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• LPS- or peptidoglycan-stimulated reactive oxygen intermediate (ROI) production by neutrophils is blocked compared to in similarly treated wild-type cells
• however, ROI production in response to PMA stimulation is normal
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• NK cells exhibit reduced conjugation with RMA-S cells compared with wild-type cells
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• LPS-stimulated bone marrow-derived macrophages fail to generate oxidative burst unlike similarly treated wild-type cells
• however, mice exhibit normal bone marrow-derived macrophages response to PMA stimulation and reactive nitrogen and prostaglandin production in response to LPS
• despite normal phagocytosis, bone marrow-derived macrophages stimulated with unopsonized heat-killed E. coli fail to generate reactive oxygen intermediate unlike similarly treated wild-type cells
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• from LPS-stimulated bone marrow-derived macrophages
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• from LPS-stimulated bone marrow-derived macrophages
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• LPS- or peptidoglycan-stimulated reactive oxygen intermediate (ROI) production by neutrophils is blocked compared to in similarly treated wild-type cells
• however, ROI production in response to PMA stimulation is normal
|
|
• NK cells exhibit reduced conjugation with RMA-S cells compared with wild-type cells
|
|
• LPS-stimulated bone marrow-derived macrophages fail to generate oxidative burst unlike similarly treated wild-type cells
• however, mice exhibit normal bone marrow-derived macrophages response to PMA stimulation and reactive nitrogen and prostaglandin production in response to LPS
• despite normal phagocytosis, bone marrow-derived macrophages stimulated with unopsonized heat-killed E. coli fail to generate reactive oxygen intermediate unlike similarly treated wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• T2 cells are reduced approximately 2-fold
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• increase in the proportion of immature cells
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• numbers of transitional 1 (T1) and newly formed B cells are increased
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• immature B lineage cells accumulate in the periphery and development is arrested at a late transitional (T1/T2) stage
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• blocked at an early stage in the thymus, at the DN3 to DN4 transition
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• 2- to 3-fold reduction in mature IgMloIgDhi B cells
|
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• few peripheral T cells
|
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• 50 to 100 fold reduction relative to wild-type
|
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• anti-Ig induced calcium responses are severely disrupted in B cells
|
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• B cells fail to proliferate in response to Ig receptor stimulation
• mature B cells that are present in mutants fail to proliferate in response to stimulation with anti-Ig antibodies
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• fail to produce any anti-TNP antibodies in response to TI-2 antigen
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• fail to produce anti-TNP-specific IgG1 antibodies after immunization
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• fail to produce anti-TNP-specific IgG2b antibodies after immunization
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• fail to produce anti-TNP-specific IgG3 antibodies after immunization
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• fail to produce anti-TNP-specific IgM antibodies after immunization
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• calcium fluxes induced by anti-CD3 antibody cross-linking are completely disrupted in T cells
|
|
• T cells do not exhibit proliferative responses to stimulation with anti-CD3 with or without anti-CD28 antibodies
• T cells are unable to generate blasts when stimulated
|
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• failure to mount either T-dependent or T-independent humoral responses
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• mutant T cells do not produce IFN-gamma in response to TCR stimulation
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• mutant T cells do not produce IL-2 in response to TCR stimulation
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• the upper zone surface of epithelial cells in the cecum and ascending colon are shorted than in wild-type mice
• epithelial cell heights in the cecum and ascending colon decrease between the middle and upper zones unlike in wild-type mice
• upper zone enterocyte differentiation, as determined by marker staining, is incomplete compared to in wild-type mice
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• beyond 6 weeks, mice develop spontaneous mucosal ulcers in the cecum and colon unlike wild-type mice
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• even after 10 days, mice fail to exhibit complete healing of colonic mucosa injury unlike similarly treated wild-type mice
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• T2 cells are reduced approximately 2-fold
|
|
• increase in the proportion of immature cells
|
|
• numbers of transitional 1 (T1) and newly formed B cells are increased
|
|
• immature B lineage cells accumulate in the periphery and development is arrested at a late transitional (T1/T2) stage
|
|
• blocked at an early stage in the thymus, at the DN3 to DN4 transition
|
|
• 2- to 3-fold reduction in mature IgMloIgDhi B cells
|
|
• few peripheral T cells
|
|
• 50 to 100 fold reduction relative to wild-type
|
|
• anti-Ig induced calcium responses are severely disrupted in B cells
|
|
• B cells fail to proliferate in response to Ig receptor stimulation
• mature B cells that are present in mutants fail to proliferate in response to stimulation with anti-Ig antibodies
|
|
• fail to produce any anti-TNP antibodies in response to TI-2 antigen
|
|
• fail to produce anti-TNP-specific IgG1 antibodies after immunization
|
|
• fail to produce anti-TNP-specific IgG2b antibodies after immunization
|
|
• fail to produce anti-TNP-specific IgG3 antibodies after immunization
|
|
• fail to produce anti-TNP-specific IgM antibodies after immunization
|
|
• calcium fluxes induced by anti-CD3 antibody cross-linking are completely disrupted in T cells
|
|
• T cells do not exhibit proliferative responses to stimulation with anti-CD3 with or without anti-CD28 antibodies
• T cells are unable to generate blasts when stimulated
|
|
• 50 to 100 fold reduction relative to wild-type
|
|
• B cells fail to proliferate in response to Ig receptor stimulation
• mature B cells that are present in mutants fail to proliferate in response to stimulation with anti-Ig antibodies
|
|
• T cells do not exhibit proliferative responses to stimulation with anti-CD3 with or without anti-CD28 antibodies
• T cells are unable to generate blasts when stimulated
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• when stimulate by anti-CD3 antibodies, CD4+ T cells exhibit reduced blast population compared with similarly treated wild-type cells
• stimulation of CD4+ or CD8+ T cells with anti-CD3 and anti-CD28 antibodies, restores blast populations to levels observed in similarly treated cells from Vav1tm1Tyb
|
|
• when stimulated with anti-CD3 antibodies, T cells exhibit reduced T cell proliferation compared with similarly treated wild-type mice or single homozygotes
• when stimulated with engagement of CD3 and CD28 receptors, helper T cell proliferation is impaired compared to in similarly treated wild-type cells to the same extent as in similarly treated T cells from Vav1tm1Tyb mice while proliferation of cytotoxic T cells is only partially restored
|
|
• T cells stimulated with anti-CD3 and anti-CD28 antibodies produce less IL2 than similarly treated wild-type or Rasgrf2tm1Esn cells
|
|
• T cells stimulated with anti-CD3 or anti-CD3 and anti-CD28 antibodies produce less TNF-alpha than similarly treated wild-type or Rasgrf2tm1Esn cells
|
|
• when stimulate by anti-CD3 antibodies, CD4+ T cells exhibit reduced blast population compared with similarly treated wild-type cells
• stimulation of CD4+ or CD8+ T cells with anti-CD3 and anti-CD28 antibodies, restores blast populations to levels observed in similarly treated cells from Vav1tm1Tyb
|
|
• when stimulated with anti-CD3 antibodies, T cells exhibit reduced T cell proliferation compared with similarly treated wild-type mice or single homozygotes
• when stimulated with engagement of CD3 and CD28 receptors, helper T cell proliferation is impaired compared to in similarly treated wild-type cells to the same extent as in similarly treated T cells from Vav1tm1Tyb mice while proliferation of cytotoxic T cells is only partially restored
|
|
• when stimulated with anti-CD3 antibodies, T cells exhibit reduced T cell proliferation compared with similarly treated wild-type mice or single homozygotes
• when stimulated with engagement of CD3 and CD28 receptors, helper T cell proliferation is impaired compared to in similarly treated wild-type cells to the same extent as in similarly treated T cells from Vav1tm1Tyb mice while proliferation of cytotoxic T cells is only partially restored
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• B cells show diminished proliferation in response to Ig receptor stimulation
|
|
• T cells are unable to generate blasts when stimulated, indicating a proliferation defect
|
|
• small decrease in mature IgMloIgDhi B cells
|
|
• T cell numbers are reduced more than in single Vav1 homozygotes
• reduction in peripheral T cells
|
|
• mutant T cells do not produce IFN-gamma in response to TCR stimulation
|
|
• mutant T cells do not produce IL-2 in response to TCR stimulation
|
|
• B cells show diminished proliferation in response to Ig receptor stimulation
|
|
• T cells are unable to generate blasts when stimulated, indicating a proliferation defect
|
|
• small decrease in mature IgMloIgDhi B cells
|
|
• T cell numbers are reduced more than in single Vav1 homozygotes
• reduction in peripheral T cells
|
|
• B cells show diminished proliferation in response to Ig receptor stimulation
|
|
• T cells are unable to generate blasts when stimulated, indicating a proliferation defect
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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