cellular
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• normal numbers of oogonia are present at birth but oocyte numbers decline rapidly after birth
• at P4 and P8 only 10% - 20% of the normal number of c-kit positive oocytes are seen
• at 8 weeks of age oocyte depletion is more pronounced
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• absence of epididymal sperm
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reproductive system
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• at 8 weeks of age ovarian dysgenesis is apparent
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• at P13 the cortex of the ovary is nearly devoid of primordial follicles
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• at P13, after the first cohort of oocytes is normally recruited to grow, the number of growing follicles is reduced 2 fold
• at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen
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• slightly smaller compared to wild-type mice
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• at 10 and 14 weeks of age testis weights are 20% - 50% of wild-type or heterozygous littermates
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• at E15.5 most oocytes appear to be in the leptotene or early zygotene stage
• at E16.5 most oocytes still have an early zygotene morphology with a few reaching an abnormal zygotene like stage with long axial elements in synapsis but with partner exchanges
• at E17.5 most nuclei are either in an abnormal zygotene like stage or have appear to be fully synapsed but lack Scp1 expression
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• normal numbers of oogonia are present at birth but oocyte numbers decline rapidly after birth
• at P4 and P8 only 10% - 20% of the normal number of c-kit positive oocytes are seen
• at 8 weeks of age oocyte depletion is more pronounced
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• Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination
• oocytes display defects in synapsis
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• increase in the incidence of apoptotic cells in the tubules
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• absence of epididymal sperm
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• few or no cells progress through meiosis
• Dcm1/Rad51 foci are nearly absent suggesting mice are unable to initiate meiotic recombination
• spermatocytes are seen with much longer axial elements and short synaptonemal complexes, with longer synaptonemal complexes but axial elements involved in synapsis with several partners, or without synaptonemal complexes
• spermatocyte nuclei with complete synapsis are not seen
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• seminiferous tubules generally contain only spermatogonia and Sertoli cells or spermatogonia and Sertoli cells plus either early prophase spermatocytes or cells with highly condensed chromatin fragments
• no spermatocytes with pachytene or later morphology are seen
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immune system
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N |
• despite expression patterns and the known role in double strand breaks in meiosis, no abnormalities in somatic hypermutation or class switch recombination are detected
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endocrine/exocrine glands
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• at 8 weeks of age ovarian dysgenesis is apparent
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• at P13 the cortex of the ovary is nearly devoid of primordial follicles
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• at P13, after the first cohort of oocytes is normally recruited to grow, the number of growing follicles is reduced 2 fold
• at 10 weeks of age only a few intermediate stage follicles and a single preovulatory follicle are seen
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• slightly smaller compared to wild-type mice
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• at 10 and 14 weeks of age testis weights are 20% - 50% of wild-type or heterozygous littermates
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Analysis Tools