Phenotypes associated with this allele

• Allele Symbol: Casp8^tm1.1Yuan
• Allele Name: targeted mutation 1.1, Junying Yuan
• Allele ID: MGI:2176089
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Casp8^tm1.1Yuan/Casp8^tm1.1Yuan	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6 * MF1	MGI:3706520
cn2	Casp8^tm1Wll/Casp8^tm1.1Yuan Tg(Mx1-cre)1Cgn/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * MF1	MGI:3055107
cn3	Casp8^tm1Wll/Casp8^tm1.1Yuan Tg(Alb1-cre)7Gsc/0	involves: 129S1/Sv * 129X1/SvJ * MF1	MGI:3055104
cn4	Casp8^tm1Wll/Casp8^tm1.1Yuan Tg(Tie1-cre)9Ref/0	involves: 129S1/Sv * 129X1/SvJ * MF1	MGI:3055108
cn5	Casp8^tm1Wll/Casp8^tm1.1Yuan Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129S1/Sv * 129X1/SvJ * MF1	MGI:3055111

Genotype
MGI:3706520

hm1

• Allelic Composition: Casp8^tm1.1Yuan/Casp8^tm1.1Yuan
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6 * MF1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:49459 )

• homozygotes exhibit no differences from homozygous Casp8^tm1Yuan mutants in the kinetics of prenatal death, however no data is presented in J:49459

cardiovascular system

abnormal myocardium layer morphology ( J:49459 )

abnormal blood circulation ( J:49459 )

• homozygotes show no differences in the gross phenotype from homozygous Casp8^tm1Yuan, however no data is presented in J:49459

growth/size/body

decreased embryo size ( J:49459 )

embryo

decreased embryo size ( J:49459 )

hematopoietic system

abnormal hematopoietic system morphology/development ( J:49459 )

muscle

abnormal myocardium layer morphology ( J:49459 )

Genotype
MGI:3055107

cn2

• Allelic Composition: Casp8^tm1Wll/Casp8^tm1.1Yuan; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * MF1

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:92722 )

• unlike mice that do not carry Casp8^tm1.1Yuan, compound heterozygous mutants survive injection with an anti-Fas Ab

hematopoietic system

abnormal myeloblast morphology/development ( J:92722 )

• 2 days after induction of cre expression the ability of progenitor cells to form myeloid or B lymphoid colonies in culture or in transplant studies is significantly decreased

• the ability of these progenitors to differentiate into macrophages is also decreased after induction of cre expression

Genotype
MGI:3055104

cn3

• Allelic Composition: Casp8^tm1Wll/Casp8^tm1.1Yuan; Tg(Alb1-cre)7Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * MF1

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:92722 )

• unlike mice that do not carry Casp8^tm1.1Yuan, compound heterozygous mutants survive after injection with an anti-Fas Ab

homeostasis/metabolism

abnormal response/metabolism to endogenous compounds ( J:92722 )

• unlike mice that do not carry Casp8^tm1.1Yuan, compound heterozygous mutants survive and do not display liver damage after injection with an anti-Fas Ab

Genotype
MGI:3055108

cn4

• Allelic Composition: Casp8^tm1Wll/Casp8^tm1.1Yuan; Tg(Tie1-cre)9Ref/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * MF1

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:92722 )

• compound heterozygotes die around E12, similar to Casp8^tm1.1Yuan homozygotes

cardiovascular system

abnormal vitelline vasculature morphology ( J:92722 )

• the density of the vasculature in the yolk sac is decreased

visceral vascular congestion ( J:92722 )

• severe congestion of the liver and large vessels of the chest and abdomen similar to Casp8^tm1.1Yuan homozygotes is seen

myocardium necrosis ( J:92722 )

• extensive necrosis of the cardiomyocytes in the ventricles and atria is seen

globular heart ( J:92722 )

• the heart has a globose shape

thin ventricular wall ( J:92722 )

• around E12 thin and occasionally ruptured ventricular walls are seen

• heart defects are not seen in mutants at E10-E11.5

pericardial edema ( J:92722 )

• around E12, the pericardial space is enlarged consistent with pericardial edema

muscle

myocardium necrosis ( J:92722 )

• extensive necrosis of the cardiomyocytes in the ventricles and atria is seen

embryo

abnormal vitelline vasculature morphology ( J:92722 )

• the density of the vasculature in the yolk sac is decreased

wavy neural tube ( J:92722 )

• an undulated neural tube is seen

abnormal visceral yolk sac morphology ( J:92722 )

• the density of the vasculature and amount of blood in the yolk sac is decreased

• increased cell death is seen in the yolk sac at E10.5 even when no signs of vascular degeneration were detected

hematopoietic system

hematopoietic system phenotype ( J:92722 )

N • hematopoietic progenitor numbers are not decreased unlike in Casp8^tm1.1Yuan homozygotes

homeostasis/metabolism

pericardial edema ( J:92722 )

• around E12, the pericardial space is enlarged consistent with pericardial edema

nervous system

wavy neural tube ( J:92722 )

• an undulated neural tube is seen

Genotype
MGI:3055111

cn5

• Allelic Composition: Casp8^tm1Wll/Casp8^tm1.1Yuan; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * MF1

hematopoietic system

abnormal macrophage differentiation ( J:92722 )

• the number monocyte progenitors is not decreased; however, recombined progenitors can not differentiate into macrophages

• the ability of bone marrow progenitors to differentiate into granulocytes is not impaired

immune system

abnormal macrophage differentiation ( J:92722 )

• the number monocyte progenitors is not decreased; however, recombined progenitors can not differentiate into macrophages

• the ability of bone marrow progenitors to differentiate into granulocytes is not impaired

cellular

abnormal macrophage differentiation ( J:92722 )

• the number monocyte progenitors is not decreased; however, recombined progenitors can not differentiate into macrophages

• the ability of bone marrow progenitors to differentiate into granulocytes is not impaired